Immunomodulatory Effects of Galectin-1 on an IgE-Mediated Allergic Conjunctivitis Model

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dc.contributor.authorMello, Claudia Bosnic [UNESP]
dc.contributor.authorRamos, Lisandra
dc.contributor.authorGimenes, Alexandre Dantas
dc.contributor.authorMoraes Andrade, Teresa Raquel de
dc.contributor.authorOliani, Sonia Maria [UNESP]
dc.contributor.authorGil, Cristiane Damas [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2015-10-21T21:22:38Z
dc.date.available2015-10-21T21:22:38Z
dc.date.issued2015-02-01
dc.description.abstractPURPOSE. Galectin (Gal)-1, a lectin found at sites of immune privilege with critical role in the inflammation, has been poorly investigated in the ocular inflammatory diseases. Here, we evaluated the therapeutic potential of Gal-1 in ocular allergy using a model of ovalbumin (OVA)-induced AC.METHODS. OVA-immunized BALB/c male mice were challenged with eye drops containing OVA on days 14 through 16 with a subset of animals pretreated intraperitoneally with recombinant Gal-1 (rGal-1) or dexamethasone (Dex).RESULTS. Recombinant Gal-1 and Dex administration on days 14 through 16 was effective in reducing the clinical signs of allergic conjunctivitis (AC), plasma anti-OVA IgE levels, Th2 (IL-4 and IL-13), and eotaxin/RANTES levels in the lymph nodes. Four hours after the last OVA challenge, rGal-1 markedly increased Gal-1 endogenous levels in the conjunctiva, and provoked eosinophilia, which persisted at 24 hours. Recombinant Gal-1 had no effect on eosinophil activation, as evidenced by the similar pattern of peroxidase eosinophil expression between cells of rGal-1-treated and untreated AC groups. Conjunctival migrated eosinophils and neutrophils exhibited high levels of Gal-1 and b2-integrin, with points of colocalization, in the rGal-1-treated groups. These different effects observed for rGal-1 were correlated with elevated levels of activated ERK and p38 at 4 hours, and diminished levels of activated JNK and p38 at 24 hours in the eyes.CONCLUSIONS. Gal-1 has an important role in ocular allergic inflammation and represents a potential target for the development of new therapeutic strategies in eye diseases.en
dc.description.affiliationUniv Estadual Paulista, UNESP, Dept Biol, Lab Imunomorfol, Sao Paulo, Brazil
dc.description.affiliationUNIFESP Univ Fed Sao Paulo, Dept Morfol &Genet, Histol Lab, BR-04023900 Sao Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, Dept Biol, Lab Imunomorfol, Sao Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 2011/21845-3
dc.description.sponsorshipIdCNPq: 476136/2011-3
dc.description.sponsorshipIdCNPq: 302768/2010-6
dc.format.extent693-704
dc.identifierhttp://iovs.arvojournals.org/article.aspx?articleid=2212823
dc.identifier.citationInvestigative Ophthalmology &visual Science, v. 56, n. 2, p. 693-704, 2015.
dc.identifier.doi10.1167/iovs.14-15100
dc.identifier.issn0146-0404
dc.identifier.lattes5102737730539655
dc.identifier.urihttp://hdl.handle.net/11449/129565
dc.identifier.wosWOS:000352137300002
dc.language.isoeng
dc.publisherAssoc Research Vision Ophthalmology Inc
dc.relation.ispartofInvestigative Ophthalmology &visual Science
dc.relation.ispartofjcr3.388
dc.relation.ispartofsjr2,058
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjecteosinophilen
dc.subjectbeta 2-integrinen
dc.subjectocular allergyen
dc.subjectTh2 cytokinesen
dc.subjectultrastructural immunogold labellingen
dc.subjectmitogen-activated protein kinasesen
dc.titleImmunomodulatory Effects of Galectin-1 on an IgE-Mediated Allergic Conjunctivitis Modelen
dc.typeArtigo
dcterms.rightsHolderAssoc Research Vision Ophthalmology Inc
unesp.author.lattes5102737730539655
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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