Expression profiles of fetal membrane nicotinamide adenine dinucleotide phosphate oxidases (NOX) 2 and 3 differentiates spontaneous preterm birth and pPROM pathophysiologies

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dc.contributor.authorPolettini, J. [UNESP]
dc.contributor.authorSilva, Marcia Guimarães da [UNESP]
dc.contributor.authorKacerovsky, M.
dc.contributor.authorSyed, T. A.
dc.contributor.authorSaade, G.
dc.contributor.authorMenon, R.
dc.contributor.institutionUniv Texas Med Branch
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Hosp Hradec Kralove
dc.contributor.institutionCharles Univ Prague
dc.date.accessioned2014-12-03T13:10:36Z
dc.date.available2014-12-03T13:10:36Z
dc.date.issued2014-03-01
dc.description.abstractIntroduction: Nicotinamide adenine dinucleotide phosphate oxidases (NOX 1-5) are enzymes that generate cellular reactive oxygen species (ROS) besides mitochondria and might be important ROS sources associated with pregnancy complications, particularly preterm premature rupture of membranes (pPROM), that has been related to ROS.Objective: To characterize NOX enzymes expression in human fetal membranes.Methods: Differential expression and localization of NOX isoforms in human fetal membranes collected from women with uncomplicated pregnancies at term, preterm birth (PTB) or pPROM and in vitro in normal term membranes maintained in an organ explant system stimulated with water-soluble cigarette smoke extract (wsCSE) were documented by real time PCR and immunohistochemistry.Results: Fetal membranes from term deliveries, PTB and pPROM expressed NOX 2, 3 and 4 mRNAs whereas NOX 1 and 5 were not detected. NOX 2 expression was 2.3-fold higher in PTB than pPROM (p = 0.005) whereas NOX 3 was 2.2-fold higher in pPROM compared to PTB (p = 0.04). NOX 2 and 3 expressions at term mimicked pPROM and PTB, respectively. No difference in NOX 4 expression was observed among the studied groups. NOX 2, 3 and 4 were localized to both amniotic and chorionic cells. Expression of NOX 2, 3 and 4 were not significant in wsCSE-stimulated membranes compared to untreated controls.Discussion/conclusions: NOX enzymes are present in the fetal membranes and are differentially expressed in PTB and pPROM. Absence of any changes in NOXs expression after wsCSE stimulation suggests ROS generation in the membranes does not always correlate with NOX expression. (C) 2014 Elsevier Ltd. All rights reserved.en
dc.description.affiliationUniv Texas Med Branch, Dept Obstet & Gynecol, Div Maternal Fetal Med Perinatal Res, Galveston, TX 77555 USA
dc.description.affiliationUNESP Univ Estadual Paulista, Dept Pathol, Botucatu Med Sch, Sao Paulo, Brazil
dc.description.affiliationUniv Hosp Hradec Kralove, Biomed Res Ctr, Hradec Kralove, Czech Republic
dc.description.affiliationCharles Univ Prague, Dept Obstet & Gynecol, Fac Med, Hradec Kralove, Czech Republic
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Dept Pathol, Botucatu Med Sch, Sao Paulo, Brazil
dc.description.sponsorshipUTMB
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdCAPES: CAPES/PGCI 12415-12-0
dc.format.extent188-194
dc.identifierhttp://dx.doi.org/10.1016/j.placenta.2013.12.012
dc.identifier.citationPlacenta. London: W B Saunders Co Ltd, v. 35, n. 3, p. 188-194, 2014.
dc.identifier.doi10.1016/j.placenta.2013.12.012
dc.identifier.issn0143-4004
dc.identifier.lattes4940791909535775
dc.identifier.lattes4940791909535775
dc.identifier.urihttp://hdl.handle.net/11449/112296
dc.identifier.wosWOS:000333495900006
dc.language.isoeng
dc.publisherW B Saunders Co Ltd
dc.relation.ispartofPlacenta
dc.relation.ispartofjcr2.434
dc.relation.ispartofsjr1,223
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectReactive species of oxygenen
dc.subjectOxidase enzymesen
dc.subjectPreterm birthen
dc.subjectPreterm premature rupture of membranesen
dc.subjectCigarette smoking extracten
dc.titleExpression profiles of fetal membrane nicotinamide adenine dinucleotide phosphate oxidases (NOX) 2 and 3 differentiates spontaneous preterm birth and pPROM pathophysiologiesen
dc.typeArtigo
dcterms.rightsHolderW B Saunders Co Ltd
unesp.author.lattes4940791909535775
unesp.author.lattes4940791909535775
unesp.author.orcid0000-0002-6719-8715[1]
unesp.author.orcid0000-0001-9858-7900[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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