Recreating the Follicular Environment: A Customized Approach for In Vitro Culture of Bovine Oocytes Based on the Origin and Differentiation State

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dc.contributor.authorLuciano, Alberto Maria
dc.contributor.authorBarros, Rodrigo Garcia
dc.contributor.authorSoares, Ana Caroline Silva [UNESP]
dc.contributor.authorBuratini, Jose [UNESP]
dc.contributor.authorLodde, Valentina
dc.contributor.authorFranciosi, Federica
dc.contributor.institutionUniversity of Milan
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionReproductive Medicine Centre
dc.date.accessioned2022-05-01T03:42:47Z
dc.date.available2022-05-01T03:42:47Z
dc.date.issued2021-01-01
dc.description.abstractThe mammalian ovary is a large source of oocytes organized into follicles at various stages of folliculogenesis. However, only a limited number of them can be used for in vitro embryo production (IVEP), while most have yet to complete growth and development to attain full meiotic and embryonic developmental competence. While the in vitro growth of primordial follicles in the ovarian cortex has the potential to produce mature oocytes, it is still at an experimental stage. The population of early antral follicles (EAFs), instead, may represent a reserve of oocytes close to completing the growth phase, which might be more easily exploited in vitro and could increase the number of female gametes dedicated to IVEP. Here we present in vitro culture strategies that have been developed utilizing physiological parameters to support the specific needs of oocytes at distinct stages of differentiation, in order to expand the source of female gametes for IVEP by maximizing the attainment of fertilizable oocytes. Furthermore, these culture systems provide powerful tools to dissect the molecular processes that direct the final differentiation of the mammalian oocyte.en
dc.description.affiliationReproductive and Developmental Biology Laboratory Department of Health Animal Science and Food Safety University of Milan
dc.description.affiliationDepartment of Structural and Functional Biology Institute of Biosciences Sao Paulo State University
dc.description.affiliationBiogenesi Reproductive Medicine Centre
dc.description.affiliationUnespDepartment of Structural and Functional Biology Institute of Biosciences Sao Paulo State University
dc.format.extent1-15
dc.identifierhttp://dx.doi.org/10.1007/978-1-0716-1246-0_1
dc.identifier.citationMethods in Molecular Biology, v. 2273, p. 1-15.
dc.identifier.doi10.1007/978-1-0716-1246-0_1
dc.identifier.issn1940-6029
dc.identifier.issn1064-3745
dc.identifier.scopus2-s2.0-85101716304
dc.identifier.urihttp://hdl.handle.net/11449/233101
dc.language.isoeng
dc.relation.ispartofMethods in Molecular Biology
dc.sourceScopus
dc.subjectChromatin
dc.subjectCumulus cells
dc.subjectGap junction
dc.subjectGerminal vesicle
dc.subjectIn vitro oocyte growth
dc.subjectIntercellular communication
dc.subjectMeiotic arrest
dc.subjectOocyte
dc.subjectOogenesis
dc.subjectPrematuration
dc.titleRecreating the Follicular Environment: A Customized Approach for In Vitro Culture of Bovine Oocytes Based on the Origin and Differentiation Stateen
dc.typeCapítulo de livro

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