Imprinted gene expression in in vivo- And in vi/ro-produced bovine embryos and chorio-allantoic membranes

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dc.contributor.authorPerecin, F. [UNESP]
dc.contributor.authorMéo, S. C.
dc.contributor.authorYamazaki, W. [UNESP]
dc.contributor.authorFerreira, C. R.
dc.contributor.authorMerighe, G. K F
dc.contributor.authorMeirelles, F. V.
dc.contributor.authorGarcia, J. M. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.date.accessioned2014-05-27T11:23:55Z
dc.date.available2014-05-27T11:23:55Z
dc.date.issued2009-06-15
dc.description.abstractCloning by nuclear transfer is often associated with poor results due to abnormal nuclear reprogramming of somatic donor cells and altered gene expression patterns. We investigated the expression patterns of imprinted genes IGF2 and IGF2R in 33- to 36-day bovine embryos and chorio-allantoic membranes derived from in vivo- and in vitro-produced embryos by somatic cell nuclear transfer (SCNT), parthenogenetic activation, and in vitro fertilization (IVF). There was a lower IGF2 expression rate in the SCNT (0.19) and parthenogenetic (0.02) groups when compared to in vivo and IVF embryos (2.01; P<0.05). In the chorio-allantoic membranes, IGF2 showed a baseline expression pattern (P<0.05) in parthenotes (0.001) when compared to in vivo, IVF (3.13), and SCNT (0.98) groups. IGF2R was less expressed (P<0.05) in SCNT chorio-allantoic membranes (0.25) when compared to the in vivo group. The low expression of IGF2 in parthenogenetic embryos and chorio-allantoic membranes confirms its imprinted status in cattle. Alterations in the relative frequency of IGF2 and IGF2R transcripts were observed in SCNT-derived bovine embryos and chorio- allantoic membranes, respectively, supporting the hypothesis that abnormalities in the expression of imprinted genes are causes of the low efficiency of SCNT procedures in this species.en
dc.description.affiliationDepartamento de Medicina Veterinária Preventiva e Reprodução Animal Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista, Jaboticabal, SP
dc.description.affiliationDepartamento de Ciências Básicas Faculdade de Zootecnia e Engenharia de Alimentos Universidade de São Paulo, Pirassununga, SP
dc.description.affiliationEmbrapa Pecuária Sudeste, Sao Carlos, SP
dc.description.affiliationDepartamento de Medicina Veterinaria Preventiva e Reproducao Animal Faculdade de Ciencias Agrarias e Veterinarias Universidade Estadual Paulista, Jaboticabal, SP
dc.description.affiliationUnespDepartamento de Medicina Veterinária Preventiva e Reprodução Animal Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista, Jaboticabal, SP
dc.description.affiliationUnespDepartamento de Medicina Veterinaria Preventiva e Reproducao Animal Faculdade de Ciencias Agrarias e Veterinarias Universidade Estadual Paulista, Jaboticabal, SP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent76-85
dc.identifierhttp://dx.doi.org/10.4238/vol8-1gmr541
dc.identifier.citationGenetics and Molecular Research, v. 8, n. 1, p. 76-85, 2009.
dc.identifier.doi10.4238/vol8-1gmr541
dc.identifier.file2-s2.0-66349090503.pdf
dc.identifier.issn1676-5680
dc.identifier.lattes2251116139872527
dc.identifier.scopus2-s2.0-66349090503
dc.identifier.urihttp://hdl.handle.net/11449/132327
dc.identifier.wosWOS:000265578600009
dc.language.isoeng
dc.publisherFunpec-editora
dc.relation.ispartofGenetics and Molecular Research
dc.relation.ispartofsjr0,439
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCattle
dc.subjectEpigenetics
dc.subjectF2
dc.subjectG
dc.subjectGenomic imprinting
dc.subjectI
dc.subjectIGF2
dc.subjectNuclear transfer
dc.subjectR
dc.subjectGlyceraldehyde 3 phosphate dehydrogenase
dc.subjectGrowth factor receptor
dc.subjectMessenger RNA
dc.subjectSomatomedin A
dc.subjectSomatomedin a receptor
dc.subjectUnclassified drug
dc.subjectSomatomedin B
dc.subjectSomatomedin B receptor
dc.subjectAnimal cell
dc.subjectAnimal experiment
dc.subjectAnimal tissue
dc.subjectCell nucleus transplantation
dc.subjectChorioallantois
dc.subjectControlled study
dc.subjectCow
dc.subjectEmbryo
dc.subjectFemale
dc.subjectFertilization in vitro
dc.subjectGene activation
dc.subjectGene expression
dc.subjectGene frequency
dc.subjectGenetic transcription
dc.subjectGenome imprinting
dc.subjectIn vitro study
dc.subjectIn vivo study
dc.subjectNonhuman
dc.subjectNucleotide sequence
dc.subjectSomatic cell
dc.subjectAnimal
dc.subjectAnimal embryo
dc.subjectCattle
dc.subjectGenetics
dc.subjectMale
dc.subjectMetabolism
dc.subjectParthenogenesis
dc.subjectBos
dc.subjectBovinae
dc.subjectAnimals
dc.subjectChorioallantoic Membrane
dc.subjectEmbryo, Mammalian
dc.subjectFemale
dc.subjectFertilization in Vitro
dc.subjectGene Expression
dc.subjectGenomic Imprinting
dc.subjectInsulin-Like Growth Factor II
dc.subjectMale
dc.subjectNuclear Transfer Techniques
dc.subjectParthenogenesis
dc.subjectReceptor, IGF Type 2
dc.titleImprinted gene expression in in vivo- And in vi/ro-produced bovine embryos and chorio-allantoic membranesen
dc.typeArtigo
dcterms.licensehttp://geneticsmr.com/node/2
unesp.author.lattes2251116139872527
unesp.author.orcid0000-0003-3988-6454[4]
unesp.author.orcid0000-0003-0372-4920[6]
unesp.author.orcid0000-0003-2009-5863[1]
unesp.author.orcid0000-0003-0046-0050[2]
unesp.author.orcid0000-0002-2996-4677[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt
unesp.departmentMedicina Veterinária Preventiva e Reprodução Animal - FCAVpt

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Jaboticabal - FCAV - Faculdade de Ciências Agrárias e Veterinárias