Assessment of fetal lung maturity using analysis of amniotic fluid from ewes that gave birth prematurely and at term

Página do item simplificado
dc.contributor.authorde Souza, Natália Cristina [UNESP]
dc.contributor.authorBovino, Fernanda
dc.contributor.authorAvilla, Larissa Gabriella
dc.contributor.authorDeschk, Maurício
dc.contributor.authorAlcindo, Jefferson F.
dc.contributor.authorFink, Maria Fernanda C.B. [UNESP]
dc.contributor.authorMendes, Luiz Cláudio N. [UNESP]
dc.contributor.authorFeitosa, Francisco Leydson F. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionFundação Educacional de Andradina (FEA)
dc.contributor.institutionUniversidade Federal de Mato Grosso do Sul (UFMS)
dc.contributor.institutionUniversidade Federal de Juiz de Fora (UFJF)
dc.contributor.institutionUniversidade Federal de Juíz de Fora (UFJF)
dc.date.accessioned2021-06-25T10:58:13Z
dc.date.available2021-06-25T10:58:13Z
dc.date.issued2020-12-01
dc.description.abstractThe aim of this study was to evaluate the lung maturity of premature and full-term lambs by analyzing amniotic fluid using the following methods: Clements test, Nile blue cytology test, hematoxylin-Shorr stain, lamellar body count, and radiographic tests. The use of these methods is intended to identify high-risk newborns and provide immediate clinical intervention after birth. Altogether, 56 animals (24 ewes and 32 lambs) were included in the study and divided into 3 groups. Group I consisted of 8 ewes that were at approximately 145 days of gestation; this group delivered 10 lambs naturally. Group II consisted of 8 ewes that were at 138 days' gestation; this group delivered 11 lambs by cesarean section. Group III consisted of 8 ewes at 138 days' gestation; this group was administered intramuscular dexamethasone (16mg/animal) 36 hours prior to a cesarean section. Group III delivered11 lambs. Cytological tests were performed using a microscope with a maximum magnification of 1000x, while the Clements test was visually observed by one of the researchers. Amnioticfluid lamellar body counts were measured using transmission electron microscopy. Among the staining methods, hematoxylin-Shorr was reliable, and Group III had a greater number of orangeophilic cells when compared to Group II, probably due to corticoid administration. The Clements test showed pulmonary maturity in approximately 20% of Group I lambs and Group II showed 9.1% of bubbles; however, Group III had the highest pulmonary maturity percentage (36.4%). The lamellar bodies were measured, and all groups had sizes between 0.019 and 0.590µm. Radiographic evaluation revealed that the majority of lambs presented some level of pulmonary radiodensity, indicating an acinar pattern at birth. These results are in line with the expectations of each group. We found that the normal group showed greater pulmonary maturity, whereas Group II presented pulmonary immaturity, which is expected because this group comprised lambs born prematurely and Group III showed pulmonary.en
dc.description.affiliationGraduate Program in Animal Science Departamento de Clínica Cirurgia e Reprodução Animal Faculdade de Medicina Veterinária Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp) Campus Araçatuba, Rua Clóvis Pestana 793, Jardim Dona Amélia
dc.description.affiliationFaculdade de Ciências Agrárias Andradina (FCAA) Fundação Educacional de Andradina (FEA), Rua Amazonas 571
dc.description.affiliationUniversidade Federal de Mato Grosso do Sul (UFMS) Cidade Universitária, Av. Costa e Silva s/n, Pioneiros
dc.description.affiliationVeterinarian Universidade Federal de Juiz de Fora (UFJF) Campus Universitário, Rua José Lourenço Kelmer s/n
dc.description.affiliationUniversidade Federal de Juíz de Fora (UFJF) Campus Universitário, Rua José Lourenço Kelmer s/n
dc.description.affiliationGraduation in Veterinary Medicine Faculdade de Medicina Veterinária Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp) Campus Araçatuba, Rua Clóvis Pestana 793, Jardim Dona Amélia
dc.description.affiliationDepartamento Clínica Cirurgia e Reprodução Animal Faculdade de Medicina Veterinária Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp) Campus Araçatuba, Rua Clóvis Pestana 793, Jardim Dona Amélia
dc.description.affiliationUnespGraduate Program in Animal Science Departamento de Clínica Cirurgia e Reprodução Animal Faculdade de Medicina Veterinária Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp) Campus Araçatuba, Rua Clóvis Pestana 793, Jardim Dona Amélia
dc.description.affiliationUnespGraduation in Veterinary Medicine Faculdade de Medicina Veterinária Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp) Campus Araçatuba, Rua Clóvis Pestana 793, Jardim Dona Amélia
dc.description.affiliationUnespDepartamento Clínica Cirurgia e Reprodução Animal Faculdade de Medicina Veterinária Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp) Campus Araçatuba, Rua Clóvis Pestana 793, Jardim Dona Amélia
dc.format.extent1039-1047
dc.identifierhttp://dx.doi.org/10.1590/1678-5150-PVB-6544
dc.identifier.citationPesquisa Veterinaria Brasileira, v. 40, n. 12, p. 1039-1047, 2020.
dc.identifier.doi10.1590/1678-5150-PVB-6544
dc.identifier.fileS0100-736X2020001201039.pdf
dc.identifier.issn1678-5150
dc.identifier.issn0100-736X
dc.identifier.scieloS0100-736X2020001201039
dc.identifier.scopus2-s2.0-85104382805
dc.identifier.urihttp://hdl.handle.net/11449/207617
dc.language.isoeng
dc.relation.ispartofPesquisa Veterinaria Brasileira
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.titleAssessment of fetal lung maturity using analysis of amniotic fluid from ewes that gave birth prematurely and at termen
dc.typeArtigo

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
S0100-736X2020001201039.pdf
Tamanho:
2.9 MB
Formato:
Adobe Portable Document Format
Baixar

Coleções

Artigos - Clínica, Cirurgia e Reprodução Animal - FMVA