Expression, purification and characterization of cold shock protein A of Corynebacterium pseudotuberculosis

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dc.contributor.authorLindae, Antje [UNESP]
dc.contributor.authorEberle, Raphael J. [UNESP]
dc.contributor.authorCaruso, Icaro P. [UNESP]
dc.contributor.authorCoronado, Monika A. [UNESP]
dc.contributor.authorMoraes, Fabio R. de [UNESP]
dc.contributor.authorAzevedo, Vasco
dc.contributor.authorArni, Raghuvir K. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.date.accessioned2015-10-21T13:14:22Z
dc.date.available2015-10-21T13:14:22Z
dc.date.issued2015-08-01
dc.description.abstractThe gram-positive bacterium Corynebacterium pseudotuberculosis is the causative agent of different diseases that cause dramatically reduced yields of wool and milk, and results in weight loss, carcass condemnation and also death mainly in sheep, equids, cattle and goats and therefore globally results in considerable economical loss. Cold shock proteins are conserved in many bacteria and eukaryotic cells and they help to restore normal cell functions after cold shock in which some appear to have specific functions at normal growth temperature as well.Cold shock protein A from C pseudotuberculosis was expressed in Escherichia coli and purified. The thermal unfolding/refolding process characterized by circular dichroism, differential scanning calorimetry and NMR spectroscopy techniques indicated that the refolding process was almost completely reversible. (C) 2015 Elsevier Inc. All rights reserved.en
dc.description.affiliationUniv Estadual Paulista UNESP, Ctr Multiusuario Inovacao Biomol, Dept Fis, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil
dc.description.affiliationUniv Fed Minas Gerais, Lab Genet Celular &Mol, Inst Ciencias Biol, BR-270901 Belo Horizonte, MG, Brazil
dc.description.affiliationUnespUniv Estadual Paulista UNESP, Ctr Multiusuario Inovacao Biomol, Departamento de Física, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent15-20
dc.identifierhttp://www.sciencedirect.com/science/article/pii/S1046592815000868
dc.identifier.citationProtein Expression And Purification. San Diego: Academic Press Inc Elsevier Science, v. 112, p. 15-20, 2015.
dc.identifier.doi10.1016/j.pep.2015.04.006
dc.identifier.issn1046-5928
dc.identifier.lattes9162508978945887
dc.identifier.orcid0000-0003-2460-1145
dc.identifier.urihttp://hdl.handle.net/11449/128845
dc.identifier.wosWOS:000355572200003
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofProtein Expression And Purification
dc.relation.ispartofjcr1.338
dc.relation.ispartofsjr0,648
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectC. pseudotuberculosisen
dc.subjectCold shock proteinen
dc.subjectSpectroscopic methodsen
dc.subjectSecondary structureen
dc.subjectFolding and refolding proteinsen
dc.titleExpression, purification and characterization of cold shock protein A of Corynebacterium pseudotuberculosisen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes9162508978945887[7]
unesp.author.orcid0000-0002-0425-0352[5]
unesp.author.orcid0000-0002-4775-2280[6]
unesp.author.orcid0000-0002-8763-3884[2]
unesp.author.orcid0000-0003-2460-1145[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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Artigos - Física - IBILCE