Overproduction and properties of lipase by a wild strain of Burkholderia lata LBBIO-BL02 using chicken fat

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dc.contributor.authorOliveira, Bruno H. [UNESP]
dc.contributor.authorSantos, Rachid É. [UNESP]
dc.contributor.authorLoiola, Luiz E. A. [UNESP]
dc.contributor.authorNascimento, Valeria M. G. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T16:57:28Z
dc.date.available2018-12-11T16:57:28Z
dc.date.issued2015-06-26
dc.description.abstractIn this work, we report the overproduction of lipases by a new wild strain of Burkholderia lata (LBBIO-BL02) in submerged fermentation to seek an economically attractive bioprocess. The best fermentation medium composition was containing chicken fat (12.5 mL/L) and ammonium phosphate (15 g/L) at 35 ºC and pH 7.0, resulting in a lipase titer of 1137.82 U/mL and 2146.83 U/mg. The lipase characterization exhibited maximum activity at 55 °C and pH 8.0. The lipase retained 100, 93 and 85 % of its maximum activity at 45, 50 and 60 °C, respectively, and 78, 82 and 100 % at pH 2.2, 3 and 10, respectively. The enzyme was successfully immobilized on Celite by adsorption and showed a promising future for various organic syntheses because of its stability in organic solvents. All the results shows that Burkholderia lata LBBIO-BL02 is a superior lipase-producing bacterium and its enzyme showed good potential for industrial and biotechnology application.en
dc.description.affiliationDepartamento de Ciências Biológicas Laboratório de Biotecnologia Industrial Faculdade de Ciências e Letras UNESP - Universidade Estadual Paulista Campus de Assis
dc.description.affiliationDepartamento de Bioquímica e Microbiologia Instituto de Biociências UNESP – Universidade Estadual Paulista, Campus de Rio Claro
dc.description.affiliationLaboratório de Bioquímica e Bioprocessos Depto. de Ciências Biológicas Universidade Estadual Paulista (UNESP), Campus de Assis, Av. Dom Antônio, 2100
dc.description.affiliationUnespDepartamento de Ciências Biológicas Laboratório de Biotecnologia Industrial Faculdade de Ciências e Letras UNESP - Universidade Estadual Paulista Campus de Assis
dc.description.affiliationUnespDepartamento de Bioquímica e Microbiologia Instituto de Biociências UNESP – Universidade Estadual Paulista, Campus de Rio Claro
dc.description.affiliationUnespLaboratório de Bioquímica e Bioprocessos Depto. de Ciências Biológicas Universidade Estadual Paulista (UNESP), Campus de Assis, Av. Dom Antônio, 2100
dc.format.extent865-877
dc.identifierhttp://dx.doi.org/10.1007/s13213-014-0928-6
dc.identifier.citationAnnals of Microbiology, v. 65, n. 2, p. 865-877, 2015.
dc.identifier.doi10.1007/s13213-014-0928-6
dc.identifier.file2-s2.0-84929844255.pdf
dc.identifier.issn1869-2044
dc.identifier.issn1590-4261
dc.identifier.scopus2-s2.0-84929844255
dc.identifier.urihttp://hdl.handle.net/11449/171857
dc.language.isoeng
dc.relation.ispartofAnnals of Microbiology
dc.relation.ispartofsjr0,479
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBioprocess design
dc.subjectCelite
dc.subjectChicken fat
dc.subjectImmobilization
dc.subjectLipase
dc.subjectOverproduction
dc.titleOverproduction and properties of lipase by a wild strain of Burkholderia lata LBBIO-BL02 using chicken faten
dc.typeArtigo

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Artigos - Ciências Biológicas - FCLAS
Artigos - Bioquímica e Microbiologia - IBRC