Fibrinogen-Induced Streptococcus mutans Biofilm Formation and Adherence to Endothelial Cells

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dc.contributor.authorLombardo Bedran, Telma Blanca [UNESP]
dc.contributor.authorAzelmat, Jabrane
dc.contributor.authorSpolidorio, Denise Palomari [UNESP]
dc.contributor.authorGrenier, Daniel
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Laval
dc.date.accessioned2014-12-03T13:10:46Z
dc.date.available2014-12-03T13:10:46Z
dc.date.issued2013-01-01
dc.description.abstractStreptococcus mutans, the predominant bacterial species associated with dental caries, can enter the bloodstream and cause infective endocarditis. The aim of this study was to investigate S. mutans biofilm formation and adherence to endothelial cells induced by human fibrinogen. The putative mechanism by which biofilm formation is induced as well as the impact of fibrinogen on S. mutans resistance to penicillin was also evaluated. Bovine plasma dose dependently induced biofilm formation by S. mutans. Of the various plasma proteins tested, only fibrinogen promoted the formation of biofilm in a dose-dependent manner. Scanning electron microscopy observations revealed the presence of complex aggregates of bacterial cells firmly attached to the polystyrene support. S. mutans in biofilms induced by the presence of fibrinogen was markedly resistant to the bactericidal effect of penicillin. Fibrinogen also significantly increased the adherence of S. mutans to endothelial cells. Neither S. mutans cells nor culture supernatants converted fibrinogen into fibrin. However, fibrinogen is specifically bound to the cell surface of S. mutans and may act as a bridging molecule to mediate biofilm formation. In conclusion, our study identified a new mechanism promoting S. mutans biofilm formation and adherence to endothelial cells which may contribute to infective endocarditis.en
dc.description.affiliationState Univ Sao Paulo, Araraquara Dent Sch, Dept Oral Diag & Surg, BR-14801903 Araraquara, SP, Brazil
dc.description.affiliationUniv Laval, Oral Ecol Res Grp, Fac Dent, Quebec City, PQ G1V 0A6, Canada
dc.description.affiliationState Univ Sao Paulo, Dept Physiol & Pathol, Araraquara Dent Sch, BR-14801903 Araraquara, SP, Brazil
dc.description.affiliationUnespState Univ Sao Paulo, Araraquara Dent Sch, Dept Oral Diag & Surg, BR-14801903 Araraquara, SP, Brazil
dc.description.affiliationUnespState Univ Sao Paulo, Dept Physiol & Pathol, Araraquara Dent Sch, BR-14801903 Araraquara, SP, Brazil
dc.description.sponsorshipCanadian Institutes of Health Research
dc.format.extent8
dc.identifierhttp://dx.doi.org/10.1155/2013/431465
dc.identifier.citationBiomed Research International. New York: Hindawi Publishing Corporation, 8 p., 2013.
dc.identifier.doi10.1155/2013/431465
dc.identifier.fileWOS000325878700001.pdf
dc.identifier.issn2314-6133
dc.identifier.urihttp://hdl.handle.net/11449/112509
dc.identifier.wosWOS:000325878700001
dc.language.isoeng
dc.publisherHindawi Publishing Corporation
dc.relation.ispartofBioMed Research International
dc.relation.ispartofjcr2.583
dc.relation.ispartofsjr0,935
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleFibrinogen-Induced Streptococcus mutans Biofilm Formation and Adherence to Endothelial Cellsen
dc.typeArtigo
dcterms.rightsHolderHindawi Publishing Corporation
unesp.author.orcid0000-0003-2376-1024[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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