Lack of reliability of nanotechnology in the of free plasma DNA in samples of patients with prostate cancer

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dc.contributor.authorMoreno, Ricardo
dc.contributor.authorDelgado, Pamela O
dc.contributor.authorCoelho, Patrícia G
dc.contributor.authorMarsicano, Sarah R
dc.contributor.authorBoas, Viviane Av
dc.contributor.authorAzzalis, Ligia A
dc.contributor.authorJunqueira, Virgínia Bc
dc.contributor.authorRocha, Katya C
dc.contributor.authorDe Abreu, Luiz Carlos
dc.contributor.authorValenti, Vitor E [UNESP]
dc.contributor.authorDrezzet, Jefferson
dc.contributor.authorPereira, Edimar Cristiano
dc.contributor.authorFonseca, Fernando La
dc.contributor.institutionFaculdade de Medicina do ABC (FMABC)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:28:10Z
dc.date.available2014-05-27T11:28:10Z
dc.date.issued2013-01-16
dc.description.abstractBackground: Several studies seek biological markers that give diagnostic and degree of tumor development. The aim of this study was to validate the determination of plasma DNA using nanotechnology (Nanovue™-NV) in samples of 80 patients with prostate cancer. Methods. Blood samples of 80 patients of the Urology Ambulatory of Faculdade de Medicina do ABC with prostate cancer confirmed by anatomical-pathology criteria were analyzed. DNA extraction was performed using a GFX TM kit (Amersham Pharmacia Biotech, Inc, USA) following the adapted protocol. Plasma was subjected to centrifugation. Results: There was a big difference between the first and the second value obtained by NanoVue Only two samples had no differences between duplicates. Maximum difference between duplicates was 38 μg/mL. Average variation between 51 samples was 10.29 μg/mL, although 21 samples had differences above this average. No correlation was observed between pDNA obtained by traditional spectrophotometry and by nanotechnology. Conclusion: Determination of plasma DNA by nanotechnology was not reproducible. © 2013 Moreno et al; licensee BioMed Central Ltd.en
dc.description.affiliationLaboratório de Análises Clínicas Faculdade de Medicina Do ABC, Av. Príncipe de Gales 821, Santo André, CEP: 09060-650
dc.description.affiliationDepartamento de Ciências Biológicas Universidade Federal de São Paulo UNIFESP, Rua Prof. Artur Riedel, 275, Diadema, São Paulo 09972-270
dc.description.affiliationLaboratório de Delineamento de Estudos e Escrita Científica Departamento de Morfologia e Fisiologia Faculdade de Medicina Do ABC, Santo André, Av. Príncipe de Gales, n. 821, Santo André, CEP: 09060-650
dc.description.affiliationPrograma de Pós-Graduação em Fisioterapia Faculdade de Ciências e Tecnologia Universidade Estadual Paulista, Rua Roberto Simonsen, 305, Presidente Prudente, São Paulo 19060-900
dc.description.affiliationUnespPrograma de Pós-Graduação em Fisioterapia Faculdade de Ciências e Tecnologia Universidade Estadual Paulista, Rua Roberto Simonsen, 305, Presidente Prudente, São Paulo 19060-900
dc.identifierhttp://dx.doi.org/10.1186/1755-7682-6-2
dc.identifier.citationInternational Archives of Medicine, v. 6, n. 1, 2013.
dc.identifier.doi10.1186/1755-7682-6-2
dc.identifier.file2-s2.0-84872131878.pdf
dc.identifier.issn1755-7682
dc.identifier.scopus2-s2.0-84872131878
dc.identifier.urihttp://hdl.handle.net/11449/74387
dc.language.isoeng
dc.relation.ispartofInternational Archives of Medicine
dc.relation.ispartofsjr0,237
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectDNA
dc.subjectNanotechnology
dc.subjectNeoplams
dc.subjectProstate
dc.subjectblood level
dc.subjectblood sampling
dc.subjectcancer patient
dc.subjectcentrifugation
dc.subjectDNA determination
dc.subjectDNA extraction
dc.subjecthuman
dc.subjectmajor clinical study
dc.subjectmale
dc.subjectnanotechnology
dc.subjectprostate cancer
dc.subjectreliability
dc.subjectspectrophotometry
dc.titleLack of reliability of nanotechnology in the of free plasma DNA in samples of patients with prostate canceren
dc.typeArtigo
dcterms.licensehttp://www.biomedcentral.com/about/license
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências e Tecnologia, Presidente Prudentept

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