Evaluation of digital method for analysis of the sciatic functional index in rats

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dc.contributor.authorFranco, Rafael
dc.contributor.authorda Silva Raimundo, Soraia
dc.contributor.authorde Andrade Belo, Marco Antonio
dc.contributor.authorde Moraes, Flávio Ruas [UNESP]
dc.contributor.authorMota, Francisco Claudio Dantas
dc.contributor.authorFatoretto, Luana Alexandra
dc.contributor.institutionUNICASTELO
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-28T22:37:18Z
dc.date.available2022-04-28T22:37:18Z
dc.date.issued2011-01-01
dc.description.abstractThis work aimed to study the reproduction and description of technique for digital sampling images during rats gait and determination of the sciatic functional index (SFI), through a glass walkway to obtain shoots with a digital camera. After controlled injury by strangulation of sciatic nerve, with 3mm of length, during 30 seconds, using hemostatic forceps, a group of 32 rats was performed 24 hours before the lesion which served as control and 24 hours, 7, 14 and 21 days after injury. The tests consisted in the filming and shooting each animal in order to observe the view from below (by a mirror to 45°) and subsequently analyzed using the IMAGE-J program. Measures were taken from the lengths of the legs (right and left), and the distance between the ankle. In the analysis of IFC, values close to zero (0) suggest that the function of the sciatic nerve is still preserved and values coming from less one hundred (-100) indicate total loss of function. It was verified in this study that 24 hours before surgery the average SFI was -7.07 ± 7.88 and 24 hours after injury these values rose to an average of -77.95 ± 13.81, being about 10 times larger, where 78% of the animals showed 60 to 100% of functional loss in motor activity, demonstrated by the gradual recovery over the days analyzed, confirming the accuracy and effectiveness of the proposed methodology. These results suggest that studies can be conducted to simplify and reduce costs using the technique for digital images of footprints during rats gait in the laboratory.en
dc.description.affiliationDiscentes do Curso de Fisioterapia Universidade Camilo Castelo Branco UNICASTELO, Descalvado, SP
dc.description.affiliationLaboratório de Farmacologia e Toxicologia Animal Curso de Medicina Veterinária UNICASTELO, Descalvado, SP
dc.description.affiliationDepartamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinária FCAV- UNESP, Jaboticabal, SP
dc.description.affiliationDiscente do Curso de Medicina UNICASTELO, Descalvado, SP
dc.description.affiliationUnespDepartamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinária FCAV- UNESP, Jaboticabal, SP
dc.format.extent305-311
dc.identifier.citationBioscience Journal, v. 27, n. 2, p. 305-311, 2011.
dc.identifier.issn1981-3163
dc.identifier.issn1516-3725
dc.identifier.scopus2-s2.0-79955764398
dc.identifier.urihttp://hdl.handle.net/11449/226320
dc.language.isopor
dc.relation.ispartofBioscience Journal
dc.sourceScopus
dc.subjectAxoniotmese
dc.subjectPeripheral nerve injury
dc.subjectRat
dc.subjectSciatic functional index
dc.titleEvaluation of digital method for analysis of the sciatic functional index in ratsen
dc.titleAvaliação de método digital para análise do índice funcional do ciático em ratospt
dc.typeArtigo

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