Murine alpha-2-macroglobulin increase during inflammatory responses and tumor growth

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dc.contributor.authorIsaac, L.
dc.contributor.authorFlorido, M. P C
dc.contributor.authorFecchio, Denise [UNESP]
dc.contributor.authorSinger, L. M.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:19:46Z
dc.date.available2014-05-27T11:19:46Z
dc.date.issued1999-09-08
dc.description.abstractObjective and Design: To determine the alpha-2-macroglobulin (alpha2M) levels in mice during acute and chronic inflammatory responses. Materials and Methods: Inflammation was induced by one of the following stimuli: carrageenin, zymosan, lipopolysacharide, thioglycollate, bacilli Calmette Guerin, PPD (in pre-immunized and non-immunized animals) and tumor cells. The concentration of alpha2M was determined in plasma or peritoneal liquid by electroimmunoassay. Results: In all the treatments employed, the plasma levels of alpha2M were higher than in untreated animals. This increase varied from 9%, 24 h after injection up a maximum of 66% 72 h post-injection. When compared to animals injected only with saline, the increases were significant 48 h after treatment with either zymosan or LPS, and 72 h after treatment with either thioglycollate or carrageenin. Treatment with BCG triggers an increase in alpha2M levels after 24 h (18.60%) and 48 h (27.90%). Immunized mice presented higher levels of this protein than non-immunized animals after challenge with PPD. The growth of Ehrlich tumor cells in the peritoneal cavity was directly correlated with the local levels of alpha2M which increased 3.5 fold, 10 days after injection. Conclusions: These results strongly indicate that in mice, the concentration of alpha2M can increase during acute and chronic inflammatory reactions with kinetics dependent on the particular kind of inflammatory agent.en
dc.description.affiliationDepartamento de Imunologia Inst. de Ciê. Bioméd. Universidade de São Paulo, Av. Prof, Lineu Prestes 1730, 05508-900 São Paulo SP
dc.description.affiliationDepartamento de Patologia Faculdade de Cie. Medicas e B. de B. UNESP, SP
dc.description.affiliationUnespDepartamento de Patologia Faculdade de Cie. Medicas e B. de B. UNESP, SP
dc.format.extent446-452
dc.identifierhttp://dx.doi.org/10.1007/s000110050485
dc.identifier.citationInflammation Research, v. 48, n. 8, p. 446-452, 1999.
dc.identifier.doi10.1007/s000110050485
dc.identifier.issn1023-3830
dc.identifier.scopus2-s2.0-0032820976
dc.identifier.urihttp://hdl.handle.net/11449/65835
dc.language.isoeng
dc.relation.ispartofInflammation Research
dc.relation.ispartofjcr2.990
dc.relation.ispartofsjr1,062
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectAcute-phase proteins
dc.subjectAlpha-2-macroglobulin
dc.subjectCytokine clearance
dc.subjectProtease inhibitor
dc.subjectTumor
dc.subjectalpha 2 macroglobulin
dc.subjectBCG vaccine
dc.subjectcarrageenan
dc.subjectlipopolysaccharide
dc.subjectthioglycolic acid
dc.subjecttuberculin
dc.subjectzymosan
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectblood level
dc.subjectcell growth
dc.subjectcontrolled study
dc.subjectEhrlich ascites tumor cell
dc.subjectelectroimmunoassay
dc.subjectinflammation
dc.subjectmale
dc.subjectmouse
dc.subjectnonhuman
dc.subjectperitoneal fluid
dc.subjecttumor growth
dc.subjectAcute-Phase Reaction
dc.subjectalpha-Macroglobulins
dc.subjectAnimals
dc.subjectCarcinoma, Ehrlich Tumor
dc.subjectChronic Disease
dc.subjectImmunoassay
dc.subjectInflammation
dc.subjectMale
dc.subjectMice
dc.subjectMycobacterium bovis
dc.subjectNeoplasm Transplantation
dc.subjectPeritoneal Cavity
dc.subjectRabbits
dc.subjectTime Factors
dc.titleMurine alpha-2-macroglobulin increase during inflammatory responses and tumor growthen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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