Standartization of broth microdilution method for Mycobacterium tuberculosis

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dc.contributor.authorLeite, Clarice Queico Fujimura [UNESP]
dc.contributor.authorBeretta, Ana Laura Remédio Zeni [UNESP]
dc.contributor.authorAnno, Ivone Shizuko [UNESP]
dc.contributor.authorTelles, Maria Alice da Silva
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionInstituto Adolfo Lutz (IAL)
dc.date.accessioned2014-05-20T14:19:59Z
dc.date.available2014-05-20T14:19:59Z
dc.date.issued2000-01-01
dc.description.abstractIndirect drug susceptibility tests of Mycobacterium tuberculosis was done to investigate the accuracy and feasibility of a broth microdilution method (BMM) for determining minimal inhibitory concentrations of conventional drugs against M. tuberculosis. Test drugs included isoniazid (H), rifampicin (R), ethambutol (E), streptomycin (S) and pyrazinamide (Z). Fifty isolates of M. tuberculosis from patients who had never received drug therapy, and H37Rv strain for control, were evaluated in the system. When comparing this method with the gold standard proportional method in Lowenstein-Jensen medium, sensitivity of 100% for all drugs and specifities of 91, 100, 96, 98 and 85% were observed respectively for H, R, E, S and Z. The BMM was read faster (14-20 days) than the proportional method (20-28 days). The microdilution method evaluated allows the testing of multiple drugs in multiple concentrations. It is easy to perform and does not require special equipment or expensive supplies. In contrast to radiometric method it does not use radioactive material.en
dc.description.affiliationUnesp
dc.description.affiliationInstituto Adolfo Lutz
dc.description.affiliationUnespUnesp
dc.format.extent127-129
dc.identifierhttp://dx.doi.org/10.1590/S0074-02762000000100021
dc.identifier.citationMemórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 95, n. 1, p. 127-129, 2000.
dc.identifier.doi10.1590/S0074-02762000000100021
dc.identifier.fileS0074-02762000000100021.pdf
dc.identifier.issn0074-0276
dc.identifier.lattes2114570774349859
dc.identifier.scieloS0074-02762000000100021
dc.identifier.urihttp://hdl.handle.net/11449/25998
dc.language.isoeng
dc.publisherInstituto Oswaldo Cruz, Ministério da Saúde
dc.relation.ispartofMemórias do Instituto Oswaldo Cruz
dc.relation.ispartofjcr2.833
dc.relation.ispartofsjr1,172
dc.rights.accessRightsAcesso aberto
dc.sourceSciELO
dc.subjectbroth microdilution methoden
dc.subjectMycobacterium tuberculosisen
dc.subjectdrugs susceptibility testen
dc.titleStandartization of broth microdilution method for Mycobacterium tuberculosisen
dc.typeArtigo
unesp.author.lattes2114570774349859
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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Artigos - Ciências Biológicas - FCFAR