Expression of protease nexin-1 and plasminogen activators during follicular growth and the periovulatory period in cattle

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dc.contributor.authorCao, M. J.
dc.contributor.authorBuratini, J.
dc.contributor.authorLussier, J. G.
dc.contributor.authorCarriere, P. D.
dc.contributor.authorPrice, C. A.
dc.contributor.institutionUniv Montreal
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:49:46Z
dc.date.available2014-05-20T13:49:46Z
dc.date.issued2006-01-01
dc.description.abstractExtracellular matrix remodeling occurs during ovarian follicular development, mediated by plasminogen activators (PAs) and PA inhibitors including protease nexin-1 (PN-1). In the present study we measured expression/activity of the PA system in bovine follicles at different stages of development by timed collection of ovaries during the first follicular wave and during the periovulatory period, and in follicles collected from an abattoir. The abundance of mRNA encoding PN-1, tissue-type PA (tPA), urokinase (uPA) and PA inhibitor-1 (PAI-1) were initially upregulated by human chorionic gonadotropin (hCG) in bovine preovulatory follicular wall homogenates. PN-1, PAI-1 and tPA mRNA expression then decreased near the expected time of ovulation, whereas uPA mRNA levels remained high. PN-1 concentration in follicular fluid (FF) decreased and reached the lowest level at the time of ovulation, whereas plasmin activity in FF increased significantly after hCG. Follicles collected from the abattoir were classified as non-atretic, early-atretic or atretic based on FF estradiol and progesterone content: PN-1 protein levels in FF were significantly higher in non-atretic than in atretic follicles, and plasmin activity was correspondingly higher in the atretic follicles. No changes in PN-1 levels in FF were observed during the growth of pre-deviation follicles early in a follicular wave. These results indicate that PN-1 may be involved in the process of atresia in non-ovulatory dominant follicles and the prevention of precocious proteolysis in periovulatory follicles.en
dc.description.affiliationUniv Montreal, Fac Med Vet, CRRA, St Hyacinthe, PQ, Canada
dc.description.affiliationUniv Estadual Paulista, Dept Fisiol, Inst Biociencias, Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Fisiol, Inst Biociencias, Botucatu, SP, Brazil
dc.format.extent125-137
dc.identifierhttp://dx.doi.org/10.1530/rep.1.00849
dc.identifier.citationReproduction. Bristol: Bio Scientifica Ltd, v. 131, n. 1, p. 125-137, 2006.
dc.identifier.doi10.1530/rep.1.00849
dc.identifier.issn1470-1626
dc.identifier.urihttp://hdl.handle.net/11449/17753
dc.identifier.wosWOS:000235243200014
dc.language.isoeng
dc.publisherBio Scientifica Ltd
dc.relation.ispartofReproduction
dc.relation.ispartofjcr3.086
dc.relation.ispartofsjr1,322
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleExpression of protease nexin-1 and plasminogen activators during follicular growth and the periovulatory period in cattleen
dc.typeArtigo
dcterms.licensehttp://www.eje-online.org/site/misc/archiving_policy.xhtml
dcterms.rightsHolderBio Scientifica Ltd
unesp.author.orcid0000-0002-6727-8280[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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Artigos - Fisiologia - IBB