Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci

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dc.contributor.authorMantellatto, Aline Meira Bonfim [UNESP]
dc.contributor.authorCaparroz, Renato
dc.contributor.authorChristofoletti, Maurício Durante [UNESP]
dc.contributor.authorPiovezan, Ubiratan
dc.contributor.authorDuarte, José Maurício Barbanti [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de Brasília (UnB)
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.date.accessioned2018-12-11T17:35:02Z
dc.date.available2018-12-11T17:35:02Z
dc.date.issued2017-10-01
dc.description.abstractThe pampas deer (Ozotoceros bezoarticus) is close to being classified as globally threatened’, with the largest population occurring in the Brazilian Pantanal. Since capture is stressful to these animals, non-invasive sampling methods such as the use of feces can provide reliable sources of DNA. The aim of this study was to use fecal samples to evaluate the genetic variability of the Brazilian Pantanal population of pampas deer. Six heterologous microsatellite markers were used to screen 142 stool specimens. Seventy-four deer were identified, of which 50 adults were used to determine the genetic characteristics of the population. The Pantanal population showed high genetic diversity (mean number of alleles per locus = 11.5, expected heterozygosity = 0.75). This is the first investigation to characterize a South American deer species using fecal DNA and demonstrates the usefulness and efficiency of this approach, as well as the feasibility of obtaining information that could not have been easily obtained by traditional DNA sampling. Our findings suggest that management strategies for this species may be much more effective if applied now when the population still shows high genetic variability.en
dc.description.affiliationNúcleo de Pesquisa e Conservação de Cervídeos Departamento de Zootecnia Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP)
dc.description.affiliationDepartamento de Genética e Morfologia Instituto de Ciências Biológicas Universidade de Brasília
dc.description.affiliationCentro de Pesquisas Agropecuárias do Pantanal (CPAP), Embrapa
dc.description.affiliationUnespNúcleo de Pesquisa e Conservação de Cervídeos Departamento de Zootecnia Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2009/04253-5
dc.format.extent774-780
dc.identifierhttp://dx.doi.org/10.1590/1678-4685-gmb-2016-0323
dc.identifier.citationGenetics and Molecular Biology, v. 40, n. 4, p. 774-780, 2017.
dc.identifier.doi10.1590/1678-4685-gmb-2016-0323
dc.identifier.fileS1415-47572017000500774.pdf
dc.identifier.issn1678-4685
dc.identifier.issn1415-4757
dc.identifier.scieloS1415-47572017000500774
dc.identifier.scopus2-s2.0-85035795479
dc.identifier.urihttp://hdl.handle.net/11449/179401
dc.language.isoeng
dc.relation.ispartofGenetics and Molecular Biology
dc.relation.ispartofsjr0,638
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectConservation
dc.subjectFecal DNA
dc.subjectMicrosatellites
dc.subjectNon-invasive methods
dc.subjectPampas deer
dc.titleGenetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite locien
dc.typeArtigo

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