Rubus rosifolius (Rosaceae) stem extract induces cell injury and apoptosis in human hepatoma cell line

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dc.contributor.authorDe Quadros, Ana Paula Oliveira [UNESP]
dc.contributor.authorOshiiwa, Bruna [UNESP]
dc.contributor.authorPetreanu, Marcel
dc.contributor.authorNiero, Rivaldo
dc.contributor.authorRosa, Paulo Cesar Pires
dc.contributor.authorSawaya, Alexandra Christine Helena Frankland
dc.contributor.authorMantovani, Mario Sergio
dc.contributor.authorO'Neill De Mascarenhas Gaivão, Isabel
dc.contributor.authorMaistro, Edson Luis [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionVale do Itajaí University (UNIVALI)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionCECAV and Department of Genetics and Biotechnology
dc.date.accessioned2023-07-29T15:13:11Z
dc.date.available2023-07-29T15:13:11Z
dc.date.issued2023-02-01
dc.description.abstractRubus rosifolius, popularly known as “red mulberry”, is a common medicinal plant in southern Brazil and is used as an antidiarrheal, analgesic, antimicrobial and antihypertensive, and to treat stomach diseases. The aim of this study was to analyze the R. rosifolius stem extract (RrSE) for possible in vitro cytotoxic and genotoxic effects, using the comet assay and the micronucleus test to assess genotoxicity, and flow cytometry to assess the impact on the cell cycle and apoptosis in HepG2/C3A cells, in addition to evaluating the expression of genes linked to the induction of DNA damage, cell cycle, apoptosis and metabolism of xenobiotics. The MTT assay observed no cytotoxic effects at concentrations between 0.01 and 100 μg/mL of the extract. However, genotoxic effects occurred in treatments with the extract from a 1 μg/mL concentration. Flow cytometry analysis revealed a significant increase in cells in the G2/M phase after treatment with 10 μg/mL, a decrease in cells in the G0/G1 phase in the treatment with 100 μg/mL, and a significant increase in total apoptotic cells. In the gene expression analysis, an increase in the CYP1A2 xenobiotics metabolizing gene expression was observed. Despite the promising pharmacological effects of R. rosifolius, the results revealed that the RrSE has genotoxic effect and induces apoptosis in HepG2/C3A cells, indicating danger in using this plant extract by humans.en
dc.description.affiliationSão Paulo State University UNESP Biosciences Institute Post-Graduate Program on General and Applied Biology, SP
dc.description.affiliationSão Paulo State University UNESP Faculty of Philosophy and Sciences Speech and Hearing Therapy Department, SP
dc.description.affiliationVale do Itajaí University (UNIVALI) Department of Biological Sciences, SC
dc.description.affiliationFaculty of Pharmaceutical Sciences University of Campinas, Campinas
dc.description.affiliationLondrina State University UEL Department of General Biology, PR
dc.description.affiliationUniversity of Trás-os-Montes and Alto Douro CECAV and Department of Genetics and Biotechnology
dc.description.affiliationUnespSão Paulo State University UNESP Biosciences Institute Post-Graduate Program on General and Applied Biology, SP
dc.description.affiliationUnespSão Paulo State University UNESP Faculty of Philosophy and Sciences Speech and Hearing Therapy Department, SP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 2017/24149-4
dc.description.sponsorshipIdCNPq: 303604/2021-2
dc.identifierhttp://dx.doi.org/10.1016/j.tiv.2022.105485
dc.identifier.citationToxicology in Vitro, v. 86.
dc.identifier.doi10.1016/j.tiv.2022.105485
dc.identifier.issn1879-3177
dc.identifier.issn0887-2333
dc.identifier.scopus2-s2.0-85141279142
dc.identifier.urihttp://hdl.handle.net/11449/249331
dc.language.isoeng
dc.relation.ispartofToxicology in Vitro
dc.sourceScopus
dc.subjectApoptosis analysis
dc.subjectCell cycle analysis
dc.subjectComet assay
dc.subjectGene expression
dc.subjectMicronucleus test
dc.subjectRubus rosifolius toxicity
dc.titleRubus rosifolius (Rosaceae) stem extract induces cell injury and apoptosis in human hepatoma cell lineen
dc.typeArtigo

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