Utilization of neuromuscular electrical stimulation in the acute phase of ankle immobilization at 90°: Study in rats

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dc.contributor.authorda Silva, Carlos Alberto
dc.contributor.authorZanelli, Marcial de Souza
dc.contributor.authorLuciano, Eliete [UNESP]
dc.contributor.authorArruda, Eder João
dc.contributor.authorGrassi, Daniel de Oliveira
dc.contributor.institutionUNIMEP
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:23:55Z
dc.date.available2014-05-27T11:23:55Z
dc.date.issued2009-06-25
dc.description.abstractObjective: To evaluate the skeletal muscle glycogen content and plasmatic concentration of interleukin -6 (IL-6), interleukin-4 (IL-4), interleukin-10 (IL-10) and tumor necrosis factor-alpha (TNF-α) in rats submitted to electrical stimulation sessions during the first three days of ankle immobilization at the position of 90°. Methods: Albinomale Wistar rats(3-4 months) were maintained in vivarium. conditions with food and water ad libitum, Submitted to 12 h photoperiodic cycles of light/dark, and distributed into 7 experimental groups (n = 6): control(C), immobilized 1 day(I1) immobilized 1 day and electrically stimulated(IE1) immobilized 2 days(12), immobilized 2 days and electrically stimulated(IE2), immobilized 3 days(13) and immobilized 3 days and electrically stimulated(IE3). Groups I utilized an acrylic resin orthesis model and groups electrically stimulated (IE) utilized the orthesis and a session of electrotherapy by a Dualpex 961 (biphasic quadratic pulse, 10 Hz, 0.4 ms, 5.0 mA, one 20 min session a day). After the experimental period, the rats were anesthetized with pentobarbital sodium(40 mg/kg) and a blood sample was colleted to evaluate the plasmatic concentration of interleukins by means of the radioimmunoassay method. The soleus and the white portion of the gastrocnemius muscle were colleted for glycogen reserves analysis(GLY). Other groups of rats were used to apply the glucose tolerance test(GTT) and insulin tolerance test(ITT). For statistical analysis, the Kolmogorov-Smirnov normality test followed by ANOVA and the Tukey tests were utilized, with a critical level established at 5%. Results: In ITT test, groups IE enhanced the skeletal muscle glucose uptake, but no changes were observed in GTT after the therapy session, which indicates that electrical stimulation is a sensibilizing method to augment skeletal muscle glucose uptake. The GLY reserves were reduced in I groups, which indicate that disuse altered insulin sensitivity and compromised energetic homeostasis. However. the IE groups displayed an augment in GLY content, suggesting that electrical stimulation restores the enzymatic pathways altered by immobilization. The improvement in GLY was accompanied by an elevation of the plasmatic concentration of IL-6 and TNF-α, showing the participation of these interleukins in the control of metabolic profile. Plasmatic concentrations of IL-10 were elevated only after 3 days of IE while IL-4 did not display any modifications. Conclusion: The results suggest that neuromuscular electricaf stimulation is an important toot in the maintenance of energetic, conditions of musculature submitted to immobilization, and presents multifactor mechanisms linked to interleukins action that converge to maintain the energetic equilibrium of the tissue in disuse.en
dc.description.affiliationUNIMEP, Rodovia do Açucar, km 156, Piracicaba, SP
dc.description.affiliationUNESP, Rio Claro, SP
dc.description.affiliationFACIS UNIMEP, Piracicaba, SP
dc.description.affiliationUnespUNESP, Rio Claro, SP
dc.format.extent249-257
dc.identifierhttp://connection.ebscohost.com/c/articles/41423298
dc.identifier.citationJournal of Chinese Clinical Medicine, v. 4, n. 5, p. 249-257, 2009.
dc.identifier.issn1562-9023
dc.identifier.lattes2933779830637191
dc.identifier.scopus2-s2.0-77953382000
dc.identifier.urihttp://hdl.handle.net/11449/71042
dc.language.isoeng
dc.relation.ispartofJournal of Chinese Clinical Medicine
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectElectrical stimulation
dc.subjectImmobilization
dc.subjectInterleukin
dc.subjectSkeletal muscle
dc.subjectglucose
dc.subjectglycogen
dc.subjectinsulin
dc.subjectinterleukin 10
dc.subjectinterleukin 4
dc.subjectinterleukin 6
dc.subjecttumor necrosis factor alpha
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectcontrolled study
dc.subjectelectrostimulation therapy
dc.subjectenergy metabolism
dc.subjectgastrocnemius muscle
dc.subjectglucose transport
dc.subjectglycogen analysis
dc.subjectglycogen muscle level
dc.subjectimmobilization
dc.subjectinsulin sensitivity
dc.subjectmale
dc.subjectmuscle atrophy
dc.subjectneuromuscular electrical stimulation
dc.subjectnonhuman
dc.subjectprotein blood level
dc.subjectrat
dc.subjectsoleus muscle
dc.subjecttherapy effect
dc.titleUtilization of neuromuscular electrical stimulation in the acute phase of ankle immobilization at 90°: Study in ratsen
dc.typeArtigo
unesp.author.lattes2933779830637191
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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