Selectivity in the mechanism of action of antimicrobial mastoparan peptide Polybia-MP1

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dc.contributor.authorSantos Cabrera, Marcia Perez dos [UNESP]
dc.contributor.authorBroggio Costa, Sabrina Thais [UNESP]
dc.contributor.authorSouza, Bibiana Monson de [UNESP]
dc.contributor.authorPalma, Mario Sergio [UNESP]
dc.contributor.authorRuggiero, José Roberto [UNESP]
dc.contributor.authorNeto, Joao Ruggiero [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T14:02:35Z
dc.date.available2014-05-20T14:02:35Z
dc.date.issued2008-07-01
dc.description.abstractMany potent antimicrobial peptides also present hemolytic activity, an undesired collateral effect for the therapeutic application. Unlike other mastoparan peptides, Polybia-MP1 (IDWKKLLDAAKQIL), obtained from the venom of the social wasp Polybia paulista, is highly selective of bacterial cells. The study of its mechanism of action demonstrated that it permeates vesicles at a greater rate of leakage on the anionic over the zwitterionic, impaired by the presence of cholesterol or cardiolipin; its lytic activity is characterized by a threshold peptide to lipid molar ratio that depends on the phospholipid composition of the vesicles. At these particular threshold concentrations, the apparent average pore number is distinctive between anionic and zwitterionic vesicles, suggesting that pores are similarly formed depending on the ionic character of the bilayer. To prospect the molecular reasons for the strengthened selectivity in Polybia-MP1 and its absence in Mastoparan-X, MD simulations were carried out. Both peptides presented amphipathic alpha-helical structures, as previously observed in Circular Dichroism spectra, with important differences in the extension and stability of the helix; their backbone solvation analysis also indicate a different profile, suggesting that the selectivity of Polybia-MP1 is a consequence of the distribution of the charged and polar residues along the peptide helix, and on how the solvent molecules orient themselves according to these electrostatic interactions. We suggest that the lack of hemolytic activity of Polybia-MP1 is due to the presence and position of Asp residues that enable the equilibrium of electrostatic interactions and favor the preference for the more hydrophilic environment.en
dc.description.affiliationSão Paulo State Univ UNESP, IBILCE, Dept Phys, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationSão Paulo State Univ UNESP, Inst Biosci, Ctr Studies Social Insects, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationUniv São Paulo, Inst Biomed Sci, Dept Phys & Biophys, BR-05508900 São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ UNESP, IBILCE, Dept Phys, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ UNESP, Inst Biosci, Ctr Studies Social Insects, BR-13506900 Rio Claro, SP, Brazil
dc.format.extent879-891
dc.identifierhttp://dx.doi.org/10.1007/s00249-008-0299-7
dc.identifier.citationEuropean Biophysics Journal With Biophysics Letters. New York: Springer, v. 37, n. 6, p. 879-891, 2008.
dc.identifier.doi10.1007/s00249-008-0299-7
dc.identifier.issn0175-7571
dc.identifier.lattes2901888624506535
dc.identifier.urihttp://hdl.handle.net/11449/22062
dc.identifier.wosWOS:000256823000017
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofEuropean Biophysics Journal With Biophysics Letters
dc.relation.ispartofjcr1.935
dc.relation.ispartofsjr0,604
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectselectivityen
dc.subjectcationic antimicrobial peptideen
dc.subjectHydrophobicityen
dc.subjectwasp venom mastoparanen
dc.subjectlytic activity and cooperativityen
dc.subjectconformational analysisen
dc.titleSelectivity in the mechanism of action of antimicrobial mastoparan peptide Polybia-MP1en
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.advisor.lattes2901888624506535
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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Artigos - Física - IBILCE
Rio Claro - CEIS - Centro de Estudos de Insetos Sociais