Differential proteomic analysis of Acidithiobacillus ferrooxidans cells maintained in contact with bornite or chalcopyrite: Proteins involved with the early bacterial response

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dc.contributor.authorFelicio, Ana Paula [UNESP]
dc.contributor.authorde Oliveira, Eliandre
dc.contributor.authorAntonia Odena, Maria
dc.contributor.authorGarcia, Oswaldo [UNESP]
dc.contributor.authorBertolini, Maria Celia [UNESP]
dc.contributor.authorCaldas Ferraz, Lucio Fabio
dc.contributor.authorMariscal Ottoboni, Laura Maria
dc.contributor.authorMarques Novo, Maria Teresa
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Barcelona
dc.contributor.institutionUniv Sao Francisco
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniv São Carlos
dc.date.accessioned2014-05-20T14:17:48Z
dc.date.available2014-05-20T14:17:48Z
dc.date.issued2011-03-01
dc.description.abstractAcidithiobacillus ferrooxidans is a chemoautotrophic bacterium capable of oxidizing ferrous iron or sulfides to obtain energy. Bornite and chalcopyrite are copper sulfides containing iron that present different susceptibilities to the bioleaching process. Here, the early bacterial response to these minerals was investigated using a differential proteomic approach. The protein profiles of cells kept in contact with bornite or chalcopyrite for 24 or 48 h were compared to that of cells not exposed to the minerals. Response to bornite exposure involved thirteen proteins, mainly related to energy metabolism, detoxification and protein synthesis. We detected increases in the expression levels of the proteins chaperonin, antioxidant and aldehyde dehydrogenase, as well as decreases in the expression levels of the proteins radical SAM domain, fructose-1,6-bisphosphatase, PfkB domain, heat shock HslVU. ribulose bisphosphate carboxylase and ribosomal proteins. Chalcopyrite contact led to a distinct metabolic response of the bacterium, since no significant alteration in the level of protein expression was detected. These findings could help to understand the metabolic impact in A. ferrooxidans after the initial addition of the cells to bornite or chalcopyrite during bioleaching processes. (C) 2010 Elsevier Ltd. All rights reserved.en
dc.description.affiliationUniv São Paulo, UNESP, Inst Chem, Araraquara, SP, Brazil
dc.description.affiliationUniv Barcelona, Barcelona, Spain
dc.description.affiliationUniv Sao Francisco, BR-12900000 Braganca Paulista, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Ctr Mol Biol & Genet Engn, UNICAMP, BR-13083875 Campinas, SP, Brazil
dc.description.affiliationUniv São Carlos, Dept Genet & Evolut, UFSCar, São Carlos, SP, Brazil
dc.description.affiliationUnespUniv São Paulo, UNESP, Inst Chem, Araraquara, SP, Brazil
dc.description.sponsorshipVale (Brazil)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipProteoRed for the proteomics network
dc.format.extent770-776
dc.identifierhttp://dx.doi.org/10.1016/j.procbio.2010.12.003
dc.identifier.citationProcess Biochemistry. Oxford: Elsevier B.V., v. 46, n. 3, p. 770-776, 2011.
dc.identifier.doi10.1016/j.procbio.2010.12.003
dc.identifier.issn1359-5113
dc.identifier.lattes8817669953838863
dc.identifier.urihttp://hdl.handle.net/11449/25339
dc.identifier.wosWOS:000288419900024
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofProcess Biochemistry
dc.relation.ispartofjcr2.616
dc.relation.ispartofsjr0,761
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectAcidithiobacillus ferrooxidansen
dc.subjectBorniteen
dc.subjectChalcopyriteen
dc.subjectBioleachingen
dc.subjectProteomic analysisen
dc.subjectMass spectrometryen
dc.titleDifferential proteomic analysis of Acidithiobacillus ferrooxidans cells maintained in contact with bornite or chalcopyrite: Proteins involved with the early bacterial responseen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes8817669953838863
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt

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