GaAs laser therapy reestablishes the morphology of the NMJ and nAChRs after injury due to bupivacaine

Página do item simplificado
dc.contributor.authorPissulin, Cristiane Neves Alessi [UNESP]
dc.contributor.authorde Souza Castro, Paula Aiello Tom�
dc.contributor.authorCodina, Fl�vio [UNESP]
dc.contributor.authorPinto, Carina Guidi [UNESP]
dc.contributor.authorVechetti-Junior, Ivan Jose [UNESP]
dc.contributor.authorMatheus, Selma Maria Michelin [UNESP]
dc.contributor.institutionUniversidade do Oeste Paulista (UNOESTE)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.date.accessioned2018-12-11T17:08:57Z
dc.date.available2018-12-11T17:08:57Z
dc.date.issued2017-02-01
dc.description.abstractBackground Local anesthetics are used to relieve pre- and postoperative pain, acting on both sodium channels and nicotinic acetylcholine receptors (nAChR) at the neuromuscular junction (NMJ). Bupivacaine acts as a non-competitive antagonist and has limitations, such as myotoxicity, neurotoxicity, and inflammation. Low-level laser therapy (LLLT) has anti-inflammatory, regenerative, and analgesic effects. The aim of the present study was to evaluate the effects of a gallium arsenide laser (GaAs) on the morphology of the NMJ and nAChRs after application of bupivacaine in the sternomastoid muscle. Methods Thirty-two adult male Wistar rats received injections of bupivacaine 0.5% (Bupi: right antimere) and 0.9% sodium chloride (Cl: left antimere). Next, the animals were divided into a Control group (C) and a Laser group (LLLT). The laser group received LLLT (GaAs 904nm, 50mW, 4,8J) in both antimeres for five consecutive days. After seven days, the animals were euthanized and the surface portion of the sternomastoid muscle was removed, frozen, and subjected to morphological and morphometric analyses of the NMJs (nonspecific esterase reaction), confocal laser scanning, and an ultrastructural analysis. The nAChRs were quantified by Western blotting. Results In the chloride group, the morphology and morphometry of the NMJs remained stable. The maximum diameters of the NMJs were lower in the Bupi (15.048���1.985) and LLLT/Bupi subgroups (15.456���1.983) compared to the Cl (18.502���2.058) and LLLT/Cl subgroups (19.356���2.522) (p�<�0.05). Ultrastructurally, LLLT reduced myonecrosis observed after application of bupivacaine, with recovery in the junctional folds and active zone. There was an increase in the perimeter of the LLLT/Bupi subgroup (150.33) compared to the Bupi subgroup (74.69) (p�<�0.01) observed by confocal microscopy. There was also an increase in the relative planar area of the NMJ after LBI (8.75) compared to CBupi (4.80) (p�<�0.01). An analysis of the protein expression of nAChRα1 showed no major differences in the groups studied. There was an increase in protein expression of the ε subunit after application of LLLT (13.055) compared to Bupi (0.251) (p�<�0.01). Taken together, the present experiments indicate that there was a positive association of the α and γ subunits (p�<�0.05). Conclusions These results demonstrate that LLLT at the dose used in this study reduced structural alterations in the NMJ and molecular changes in nAChRs triggered by bupivacaine, providing important data supporting the use of LLLT in therapeutic protocols for injuries triggered by local anesthetics.en
dc.description.affiliationDepartment of Anatomy Universidade do Oeste Paulista (UNOESTE)
dc.description.affiliationGeneral Bases of Surgery Botucatu Medical School UNESP
dc.description.affiliationDepartament of Physical Therapy Center for Biologic Sciences and Health – CBSH Federal University of S�o Carlos/UFSCar/S�o Carlos
dc.description.affiliationSciences Student UNESP Institute of Biosciences
dc.description.affiliationDepartment of Anatomy Institute of Biosciences S�o Paulo State University (UNESP)
dc.description.affiliationDepartment of Morphology Institute of Biosciences S�o Paulo State University (UNESP)
dc.description.affiliationDepartment of Anatomy Institute of Biosciences General Bases of Surgery Botucatu Medical School
dc.description.affiliationUNESP
dc.description.affiliationUnespGeneral Bases of Surgery Botucatu Medical School UNESP
dc.description.affiliationUnespSciences Student UNESP Institute of Biosciences
dc.description.affiliationUnespDepartment of Anatomy Institute of Biosciences S�o Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Morphology Institute of Biosciences S�o Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Anatomy Institute of Biosciences General Bases of Surgery Botucatu Medical School
dc.description.affiliationUnespUNESP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 13/26649-3
dc.format.extent256-263
dc.identifierhttp://dx.doi.org/10.1016/j.jphotobiol.2016.12.024
dc.identifier.citationJournal of Photochemistry and Photobiology B: Biology, v. 167, p. 256-263.
dc.identifier.doi10.1016/j.jphotobiol.2016.12.024
dc.identifier.file2-s2.0-85009153342.pdf
dc.identifier.issn1873-2682
dc.identifier.issn1011-1344
dc.identifier.scopus2-s2.0-85009153342
dc.identifier.urihttp://hdl.handle.net/11449/174060
dc.language.isoeng
dc.relation.ispartofJournal of Photochemistry and Photobiology B: Biology
dc.relation.ispartofsjr0,698
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBupivacaine
dc.subjectLow-level light therapy
dc.subjectNeuromuscular junction
dc.subjectNicotinic acetylcholine receptor
dc.titleGaAs laser therapy reestablishes the morphology of the NMJ and nAChRs after injury due to bupivacaineen
dc.typeArtigo
unesp.author.orcid0000-0003-0613-0140 0000-0003-0613-0140[1]

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
2-s2.0-85009153342.pdf
Tamanho:
1.55 MB
Formato:
Adobe Portable Document Format
Descrição:
Baixar

Coleções

Artigos - Anatomia - IBB