Estimation of growth fraction in fine needle aspirates from non-Hodgkin's lymphoma using anti-proliferating cell nuclear antigen - Correlation with the Kiel classification

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dc.contributor.authorSchmitt, F. C.
dc.contributor.authorRabenhorst, S. H.
dc.contributor.authorMaeda, S. A.
dc.contributor.authorColturato, V
dc.contributor.authorNiéro-Melo, Ligia [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:25:06Z
dc.date.available2014-05-20T15:25:06Z
dc.date.issued1996-03-01
dc.description.abstractOBJECTIVE: This study was undertaken to assess whether fine needle aspirates from non-Hodgkins lymphoma (NHL) could be used for growth fraction analysis with proliferating cell nuclear antigen (PCNA) staining and if there was a relationship between the growth fraction and cytomorphologic classification according to the Kiel classification.STUDY DESIGN: the study group consisted of 40 patients with NHL diagnosed by fine needle aspiration (FNA) cytology. The cytologic classification of the lymphomas was made by two cytopathologists on May-Grunwald-Giemsa-stained slides using the Kiel classification. There were 27 cases of low and 13 of high grade lymphoma. The estimation of the growth fraction was made by PCNA immunoreactivity. The PCNA index was quantitated in smears by counting an average of 1,000 cells, and the count teas correlated with the cytomorphologic classification.RESULTS: There was It strong correlation between the PCNA index and lymphoma grading. High grade lymphomas exhibited a mean PCNA positivity of 74.0%, which was significantly higher (P <.001) than that of low grade lymphomas (17.6%).CONCLUSION: Our study showed that PCNA evalua tion is suitable for smears obtained by FNA on NHL, correlates with increasing grades of lymphoma according to the Kiel classification and may offer a method of monitoring treatment of lymphoma.en
dc.description.affiliationUNIV ESTADUAL PAULISTA,SCH MED,DEPT PATHOL,BOTUCATU,SP,BRAZIL
dc.description.affiliationUNIV ESTADUAL PAULISTA,SCH MED,DEPT INTERNAL MED,BOTUCATU,SP,BRAZIL
dc.description.affiliationUNIV ESTADUAL PAULISTA,SCH MED,FINE NEEDLE AMBULATORY SERV,BOTUCATU,SP,BRAZIL
dc.description.affiliationUnespUNIV ESTADUAL PAULISTA,SCH MED,DEPT PATHOL,BOTUCATU,SP,BRAZIL
dc.description.affiliationUnespUNIV ESTADUAL PAULISTA,SCH MED,DEPT INTERNAL MED,BOTUCATU,SP,BRAZIL
dc.description.affiliationUnespUNIV ESTADUAL PAULISTA,SCH MED,FINE NEEDLE AMBULATORY SERV,BOTUCATU,SP,BRAZIL
dc.format.extent199-204
dc.identifierhttp://dx.doi.org/10.1159/000333738
dc.identifier.citationActa Cytologica. St Louis: Sci Printers & Publ Inc., v. 40, n. 2, p. 199-204, 1996.
dc.identifier.doi10.1159/000333738
dc.identifier.issn0001-5547
dc.identifier.urihttp://hdl.handle.net/11449/35573
dc.identifier.wosWOS:A1996UD40000009
dc.language.isoeng
dc.publisherSci Printers & Publ Inc
dc.relation.ispartofActa Cytologica
dc.relation.ispartofsjr0,480
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectnon-Hodgkin's lymphomapt
dc.subjectimmunocytochemistrypt
dc.subjectaspiration biopsypt
dc.subjectgrowth fractionpt
dc.titleEstimation of growth fraction in fine needle aspirates from non-Hodgkin's lymphoma using anti-proliferating cell nuclear antigen - Correlation with the Kiel classificationen
dc.typeArtigo
dcterms.licensehttp://www.karger.com/Services/RightsPermissions
dcterms.rightsHolderSci Printers & Publ Inc
unesp.author.orcid0000-0003-1006-6946[1]
unesp.author.orcid0000-0002-3711-8681[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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