Single CpG island methylation is not sufficient to maintain the silenced expression of CASPASE-8 apoptosis-related gene among women with epithelial ovarian cancer

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dc.contributor.authorBraga, Leticia da Conceicao [UNESP]
dc.contributor.authorSilva, Luciana Maria
dc.contributor.authorAlvares da Silva Ramos, Ana Paula
dc.contributor.authorPiedade, Josiane Barbosa
dc.contributor.authorTeixeira Vidigal, Paula Vieira
dc.contributor.authorTraiman, Paulo [UNESP]
dc.contributor.authorSilva Filho, Agnaldo Lopes da [UNESP]
dc.contributor.institutionDiretoria Pesquisa & Desenvolvimento Fdn Ezequiel
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-12-03T13:10:33Z
dc.date.available2014-12-03T13:10:33Z
dc.date.issued2014-02-01
dc.description.abstractDespite impressive research efforts, the biology of epithelial ovarian cancer (EOC) remains poorly understood and alterations in the expression of CASPASE-8 contribute to a worse tumor prognosis. This study assesses the methylation of the CpG island within the CASPASE-8 promoter and CASPASE-8 gene expression both in cystadenoma tumors and in primary and metastatic EOC. DNA and RNA were obtained from women with normal ovarian tissues (n = 18), ovarian serous cystadenoma tumors (n = 11) and EOC (n = 16) using Trizol (R). The methylation frequency of the CpG island in the CASPASE-8 promoter was assessed using the methylation-specific PCR assay after DNA bisulfite conversion. Quantitative PCR was performed to quantify the relative levels of CASPASE-8 in each sample. The differences between samples with each group were evaluated using the Mann-Whitney U and Kruskal-Wallis tests as indicated. Hemimethylation of the CASPASE-8 promoter was found in 11.8% of the normal ovary samples, 20% of the cystadenoma tumors and 20% of the metastatic EOC, while methylation of the CASPASE-8 promoter was absent in the EOC primary tissues (P = 0.047). An increased CASPASE-8 expression level was observed in all tumor groups. Significant differences were observed in the CASPASE-8 expression levels when compared with all ovarian tumor groups (P = 0.0278). Promoter DNA methylation did not associate with expression levels of CASPASE-8, suggesting the presence of other mechanisms in relation to gene expression control in EOC; thus providing a better understanding of this complex disease. (C) 2013 Elsevier Masson SAS. All rights reserved.en
dc.description.affiliationDiretoria Pesquisa & Desenvolvimento Fdn Ezequiel, Serv Biol Celular, Belo Horizonte, MG, Brazil
dc.description.affiliationUniv Fed Minas Gerais, Dept Patol & Med Legal, Fac Med, Belo Horizonte, MG, Brazil
dc.description.affiliationUniv Fed Minas Gerais, Dept Ginecol & Obstet, Fac Med, Belo Horizonte, MG, Brazil
dc.description.affiliationUniv Estadual Sao Paulo Julio de Mesquita Filho, Dept Ginecol & Obstet, Fac Med, Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Sao Paulo Julio de Mesquita Filho, Dept Ginecol & Obstet, Fac Med, Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipPro-Rectory of Research of the Universidade Federal de Minas Gerais
dc.description.sponsorshipIdFAPEMIG: PPM-CDS-00246-09
dc.format.extent87-91
dc.identifierhttp://dx.doi.org/10.1016/j.biopha.2013.12.004
dc.identifier.citationBiomedicine & Pharmacotherapy. Paris: Elsevier France-editions Scientifiques Medicales Elsevier, v. 68, n. 1, p. 87-91, 2014.
dc.identifier.doi10.1016/j.biopha.2013.12.004
dc.identifier.issn0753-3322
dc.identifier.lattes8334785337106990
dc.identifier.urihttp://hdl.handle.net/11449/112245
dc.identifier.wosWOS:000332448400014
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofBiomedicine & Pharmacotherapy
dc.relation.ispartofjcr3.457
dc.relation.ispartofsjr0,951
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectEpithelial ovarian canceren
dc.subjectCASPASE-8en
dc.subjectExpressionen
dc.titleSingle CpG island methylation is not sufficient to maintain the silenced expression of CASPASE-8 apoptosis-related gene among women with epithelial ovarian canceren
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes8334785337106990
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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