Effect of the bone marrow cell transplantation on elevated plus-maze performance in hippocampal-injured mice

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dc.contributor.authorDa Cruz e Alves-de-Moraes, Luís Bruno
dc.contributor.authorRibeiro-Paes, João Tadeu [UNESP]
dc.contributor.authorLongo, Beatriz Monteiro
dc.contributor.authorFerrazoli, Enéas Galdini
dc.contributor.authorAndrade, Telma Gonçalves Carneiro Spera de [UNESP]
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2014-05-27T11:29:48Z
dc.date.available2014-05-27T11:29:48Z
dc.date.issued2013-07-01
dc.description.abstractSeveral reports have shown that the hippocampus plays an important role in different aspects of the emotional control. There is evidence that lesions in this structure cause behavioral disinhibition, with reduction of reactions expressing fear and anxiety. Thus, to portray the aptitude of cell therapy to abrogate injuries of hippocampal tissue, we examined the behavioral effects of bone marrow mononuclear cells (BMMCs) transplantation on C57BL/6 mice that had the hippocampus damaged by electrolytic lesion. For this purpose, mice received, seven days after bilateral electrolytic lesion in the dorsal hippocampus, culture medium or BMMCs expressing the enhanced green fluorescent protein (EGFP) transgene. One week after transplantation, animals were tested in the elevated plus-maze (EPM). On the whole, three assessment sessions in the EPM were carried out, with seven days separating each trial. Thirty-five days after the induction of injury, mice were sacrificed and their brains removed for immunohistochemistry. The behavioral evaluation showed that the hippocampal lesion caused disinhibition, an effect which was slightly lessened, from the second EPM test, in transplanted subjects. On the other hand, immunohistochemical data revealed an insignificant presence of EGFP+ cells inside the brains of injured mice. In view of such scenario, we hypothesized that the subtle rehabilitation of the altered behavior might be a result from a paracrine effect from the transplanted cells. This might have been caused by the release of bioactive factors capable of boosting endogenous recuperative mechanisms for a partial regaining of the hippocampal functions. © 2013 Elsevier B.V.en
dc.description.affiliationPost-Graduation in Biotechnology USP/IPT/I. Butantan, Avenida Prof. Lineu Prestes 2415 Cidade Universitária, 05508-900, São Paulo, SP
dc.description.affiliationUniversidade Estadual Paulista (UNESP) FCL Department of Biological Science, Avenida Dom Antonio 2100, 19806-900, Assis, SP
dc.description.affiliationDepartment of Physiology, ECB UNIFESP, Rua Botucatu 862, 04023-062, São Paulo, SP
dc.description.affiliationUnespUniversidade Estadual Paulista (UNESP) FCL Department of Biological Science, Avenida Dom Antonio 2100, 19806-900, Assis, SP
dc.format.extent32-40
dc.identifierhttp://dx.doi.org/10.1016/j.bbr.2013.03.042
dc.identifier.citationBehavioural Brain Research, v. 248, p. 32-40.
dc.identifier.doi10.1016/j.bbr.2013.03.042
dc.identifier.issn0166-4328
dc.identifier.issn1872-7549
dc.identifier.scopus2-s2.0-84877026011
dc.identifier.urihttp://hdl.handle.net/11449/75753
dc.identifier.wosWOS:000320497700005
dc.language.isoeng
dc.relation.ispartofBehavioural Brain Research
dc.relation.ispartofjcr3.173
dc.relation.ispartofsjr1,413
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectAnxiety
dc.subjectBone marrow cells
dc.subjectCell therapy
dc.subjectElectrolytic lesion
dc.subjectElevated plus-maze
dc.subjectHippocampus
dc.subjectenhanced green fluorescent protein
dc.subjectanimal cell
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectbone marrow transplantation
dc.subjectbrain injury
dc.subjectcontrolled study
dc.subjectgene expression
dc.subjecthippocampus
dc.subjectmale
dc.subjectmaze test
dc.subjectmononuclear cell
dc.subjectmouse
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjecttherapy effect
dc.subjecttransgene
dc.titleEffect of the bone marrow cell transplantation on elevated plus-maze performance in hippocampal-injured miceen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
unesp.author.orcid0000-0002-7645-5891[2]
unesp.author.orcid0000-0002-7918-8776[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências e Letras, Assispt
unesp.departmentCiências Biológicas - FCLASpt

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