PRP8 intein in Ajellomycetaceae family pathogens: Sequence analysis, splicing evaluation and homing endonuclease activity

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dc.contributor.authorTheodoro, Raquel Cordeiro [UNESP]
dc.contributor.authorVolkmann, Gerrit
dc.contributor.authorLiu, Xiang-Qin
dc.contributor.authorBagagli, Eduardo [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionDalhousie Univ
dc.date.accessioned2014-05-20T13:51:01Z
dc.date.available2014-05-20T13:51:01Z
dc.date.issued2011-02-01
dc.description.abstractInteins are intervening sequences that are transcribed and translated with flanking host protein sequences and then self-excised by protein splicing. Bi-functional inteins also contain a homing endonuclease responsible for their genetic mobility. The PRP8 intein, the most widespread among fungi, occurs in important pathogens such as Histoplasma capsulatum and Paracoccidioides brasiliensis, from the Ajellomycetaceae family. Herein, we describe the bi-functional PRP8 intein in two other Ajellomycetacean pathogens, Blastomyces dermatitidis and Emmonsia parva. Sequence analysis and experimental evidence suggest that the homing endonuclease from PbrPRP8 is inactive. The splicing activity of the PRP8 intein from the B. dermatitidis, E. parva and P. brasiliensis species complex was demonstrated in a non-native protein context in Escherichia coil. Since the PRP8 intein is located in a functionally essential nuclear protein, it can be considered a promising therapeutic target for anti-fungal drugs, because inhibition of intein splicing should inhibit proliferation of intein-containing pathogens. (C) 2010 Published by Elsevier B.V.en
dc.description.affiliationUNESP, Dept Microbiol & Immunol, Inst Biosci, Botucatu, SP, Brazil
dc.description.affiliationDalhousie Univ, Dept Biochem & Mol Biol, Halifax, NS, Canada
dc.description.affiliationUnespUNESP, Dept Microbiol & Immunol, Inst Biosci, Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipNatural Sciences and Engineering Research Council of Canada (NSERC)
dc.description.sponsorshipIdFAPESP: 06/03597-4
dc.description.sponsorshipIdFAPESP: 07/01306-5
dc.description.sponsorshipIdCAPES: 464108-6
dc.format.extent80-91
dc.identifierhttp://dx.doi.org/10.1016/j.fgb.2010.07.010
dc.identifier.citationFungal Genetics and Biology. San Diego: Academic Press Inc. Elsevier B.V., v. 48, n. 2, p. 80-91, 2011.
dc.identifier.doi10.1016/j.fgb.2010.07.010
dc.identifier.issn1087-1845
dc.identifier.lattes3320327570429539
dc.identifier.orcid0000-0002-8003-4109
dc.identifier.urihttp://hdl.handle.net/11449/18216
dc.identifier.wosWOS:000286487600002
dc.language.isoeng
dc.publisherAcademic Press Inc. Elsevier B.V.
dc.relation.ispartofFungal Genetics and Biology
dc.relation.ispartofjcr3.476
dc.relation.ispartofsjr1,611
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectAjellomycetaceaeen
dc.subjectInteinen
dc.subjectPRP8en
dc.subjectSplicingen
dc.subjectHoming endonucleaseen
dc.titlePRP8 intein in Ajellomycetaceae family pathogens: Sequence analysis, splicing evaluation and homing endonuclease activityen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderAcademic Press Inc. Elsevier B.V.
unesp.author.lattes3320327570429539[4]
unesp.author.orcid0000-0002-8003-4109[4]
unesp.author.orcid0000-0001-5016-1046[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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Artigos - Microbiologia e Imunologia - IBB