MicroRNA signatures characterizing caste-independent ovarian activity in queen and worker honeybees (Apis mellifera L.)

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dc.contributor.authorMacedo, L. M. F.
dc.contributor.authorNunes, F. M. F.
dc.contributor.authorFreitas, F. C. P.
dc.contributor.authorPires, C. V.
dc.contributor.authorTanaka, E. D.
dc.contributor.authorMartins, J. R.
dc.contributor.authorPiulachs, M. -D.
dc.contributor.authorCristino, A. S.
dc.contributor.authorPinheiro, D. G. [UNESP]
dc.contributor.authorSimoes, Z. L. P.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.contributor.institutionUniv Pompeu Fabra
dc.contributor.institutionUniv Queensland
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-11-26T16:32:56Z
dc.date.available2018-11-26T16:32:56Z
dc.date.issued2016-06-01
dc.description.abstractQueen and worker honeybees differ profoundly in reproductive capacity. The queen of this complex society, with 200 highly active ovarioles in each ovary, is the fertile caste, whereas the workers have approximately 20 ovarioles as a result of receiving a different diet during larval development. In a regular queen-right colony, the workers have inactive ovaries and do not reproduce. However, if the queen is sensed to be absent, some of the workers activate their ovaries, producing viable haploid eggs that develop into males. Here, a deep-sequenced ovary transcriptome library of reproductive workers was used as supporting data to assess the dynamic expression of the regulatory molecules and microRNAs (miRNAs) of reproductive and nonreproductive honeybee females. In this library, most of the differentially expressed miRNAs are related to ovary physiology or oogenesis. When we quantified the dynamic expression of 19 miRNAs in the active and inactive worker ovaries and compared their expression in the ovaries of virgin and mated queens, we noted that some miRNAs (miR-1, miR-31a, miR-13b, miR-125, let-7 RNA, miR100, miR-276, miR-12, miR-263a, miR-306, miR-317, miR-92a and miR-9a) could be used to identify reproductive and nonreproductive statuses independent of caste. Furthermore, integrative gene networks suggested that some candidate miRNAs function in the process of ovary activation in worker bees.en
dc.description.affiliationUniv Sao Paulo, Dept Genet, Fac Med Ribeirao Preto, BR-14040901 Ribeirao Preto, Brazil
dc.description.affiliationUniv Fed Sao Carlos, Dept Genet & Evolucao, Ctr Ciencias Biol & Saude, BR-13560 Sao Carlos, SP, Brazil
dc.description.affiliationUniv Pompeu Fabra, CSIC, Inst Evolutionary Biol, Barcelona, Spain
dc.description.affiliationUniv Queensland, Diamantina Inst, Translat Res Inst, Brisbane, Qld, Australia
dc.description.affiliationUniv Estadual Paulista, Dept Technol, Fac Ciencias Agr & Vet, Jaboticabal, Brazil
dc.description.affiliationUniv Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, Av Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Technol, Fac Ciencias Agr & Vet, Jaboticabal, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipMinistry of Science and Innovation, Spain
dc.description.sponsorshippostdoctoral research grant JAE-Doc ('Junta para la Ampliacion de Estudios') - Consejo Superior de Investigaciones Cientificas
dc.description.sponsorshipEuropean Social Fund
dc.description.sponsorshipIdFAPESP: 2011/03171-5
dc.description.sponsorshipIdFAPESP: 2010/08150-3
dc.description.sponsorshipIdFAPESP: 2010/06336-2
dc.description.sponsorshipIdMinistry of Science and Innovation, Spain: BFU2008-00484
dc.format.extent216-226
dc.identifierhttp://dx.doi.org/10.1111/imb.12214
dc.identifier.citationInsect Molecular Biology. Hoboken: Wiley, v. 25, n. 3, p. 216-226, 2016.
dc.identifier.doi10.1111/imb.12214
dc.identifier.issn0962-1075
dc.identifier.urihttp://hdl.handle.net/11449/161488
dc.identifier.wosWOS:000375669800003
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofInsect Molecular Biology
dc.relation.ispartofsjr1,189
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectmicroRNA
dc.subjecttranscriptome
dc.subjectovary
dc.subjectplasticity
dc.subjectdevelopment
dc.subjecthoneybee
dc.titleMicroRNA signatures characterizing caste-independent ovarian activity in queen and worker honeybees (Apis mellifera L.)en
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-Blackwell

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