Anti-Mycobacterium tuberculosis activity of fungus Phomopsis stipata

Prince, Karina Andrade de [UNESP]; Sordi, Renata [UNESP]; Pavan, Fernando Rogério [UNESP]; Santos, Adolfo Carlos Barreto [UNESP]; Araujo, Angela R. [UNESP]; Leite, Sergio R.A. [UNESP]; Leite, Clarice Queico Fujimura [UNESP]

Anti-Mycobacterium tuberculosis activity of fungus Phomopsis stipata

Arquivos

S1517-83822012000100024.pdf (129.8 KB)

Data

2012-03-01

Autores

Prince, Karina Andrade de

Sordi, Renata

Pavan, Fernando Rogério

Santos, Adolfo Carlos Barreto

Araujo, Angela R.

Leite, Sergio R.A.

Leite, Clarice Queico Fujimura

Editor

Sociedade Brasileira de Microbiologia

Tipo

Artigo

Direito de acesso

Acesso aberto

Resumo

Our purpose was to determine the anti-Mycobacterium tuberculosis activity of the metabolites produced by the endophitic fungus Phomopsis stipata (Lib.) B. Sutton, (Diaporthaceae), cultivated in different media. The antimycobacterial activity was assessed through the Resazurin Microtiter Assay (REMA) and the cytotoxicity test performed on macrophage cell line. The extracts derived from fungi grown on Corn Medium and Potato Dextrose Broth presented the smallest values of Minimum Inhibitory Concentration (MIC) and low cytotoxicity, which implies a high selectivity index. This is the first report on the chemical composition and antitubercular activity of metabolites of P. stipata, as well as the influence of culture medium on these properties.