Antioxidant activity of indigo and its preventive effect against ethanol-induced DNA damage in rat gastric mucosa

Página do item simplificado
dc.contributor.authorFarias-Silva, Elisangela
dc.contributor.authorCola, Maira
dc.contributor.authorCalvo, Tamara R.
dc.contributor.authorBarbastefano, Victor
dc.contributor.authorFerreira, Anderson L.
dc.contributor.authorMichelattol, Debora de Paula
dc.contributor.authorAlves de Almeida, Ana Cristina
dc.contributor.authorHiruma-Lima, Clelia A.
dc.contributor.authorVilegas, Wagner [UNESP]
dc.contributor.authorBrito, Alba R. M. Souza
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:49:43Z
dc.date.available2014-05-20T13:49:43Z
dc.date.issued2007-10-01
dc.description.abstractEthanol-induced oxidative damage is commonly associated with the generation of reactive oxygen molecules, leading to oxidative stress. Considering that antioxidant activity is an important mechanism of action involved in cytoprotection, the aim of this work was to evaluate the antioxidant properties of the alkaloid indigo (1) (2 mg/kg, p. o.), obtained from the leaves of Indigofera truxillensis Kunth (Fabaceae), on rat gastric mucosa submitted to ethanol-induced (100%, 1 mL, p.o.) gastric ulcer. Enzymatic assays and DNA fragmentation analysis were performed. When ethanol was administered to the control group, the sulfhydryl content (SH) and the glutathione peroxidase (GPx) activity decreased by 41% and 50%, respectively; in contrast, superoxide dismutase (SOD) and glutathione reductase (GR) activities increased by 56% and 67%, respectively. Additionally, myeloperoxidase (MPO) activity, a marker for free radical generation caused by polymorphonuclear neutrophil (PMN) tissue infiltration, also increased 4.5-fold after ethanol treatment. Rat gastric mucosa exposed to ethanol showed DNA fragmentation. Indigo alkaloid pretreatment protected rats from ethanol-induced gastric lesions. This effect was determined by the ulcerative lesion area (ULA), indicating an inhibition of around 80% at 2 mg/kg. This alkaloid also diminished GPx activity, which was higher than that observed with ethanol alone. However, this effect was counterbalanced by increased GR activity. Indigo was unable to restore alterations in SOD activity promoted by ethanol. After indigo pretreatment, SH levels and MPO activity remained normal and gastric mucosa DNA damage caused by ethanol was also partially prevented by indigo. These results suggest that the gastroprotective mechanisms of indigo include non-enzymatic antioxidant effects and the inhibition of PMN infiltration which, in combination, partially protect the gastric mucosa against ethanol-induced DNA damage.en
dc.description.affiliationUniv Estadual Campinas, Dept Fisiol & Biofis, IB, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUNESP, Dept Quim Organ, Araraquara, SP, Brazil
dc.description.affiliationUNESP, IBB, Dept Fisiol, Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, Dept Quim Organ, Araraquara, SP, Brazil
dc.description.affiliationUnespUNESP, IBB, Dept Fisiol, Botucatu, SP, Brazil
dc.format.extent1241-1246
dc.identifierhttp://dx.doi.org/10.1055/s-2007-981613
dc.identifier.citationPlanta Medica. Stuttgart: Georg Thieme Verlag Kg, v. 73, n. 12, p. 1241-1246, 2007.
dc.identifier.doi10.1055/s-2007-981613
dc.identifier.issn0032-0943
dc.identifier.lattes3814504901386844
dc.identifier.orcid0000-0002-8645-3777
dc.identifier.orcid0000-0003-3032-2556
dc.identifier.urihttp://hdl.handle.net/11449/17727
dc.identifier.wosWOS:000250481000002
dc.language.isoeng
dc.publisherGeorg Thieme Verlag Kg
dc.relation.ispartofPlanta Medica
dc.relation.ispartofjcr2.494
dc.relation.ispartofsjr0,581
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectalkaloidspt
dc.subjectIndigofera truxillensispt
dc.subjectindigopt
dc.subjectFabaceaept
dc.subjectfree radicalspt
dc.subjectantioxidant enzymespt
dc.subjectgastric ulcerpt
dc.subjectMPO activitypt
dc.subjectDNA damagept
dc.titleAntioxidant activity of indigo and its preventive effect against ethanol-induced DNA damage in rat gastric mucosaen
dc.typeArtigo
dcterms.licensehttp://www.thieme.com//media/ita/pubid-1019644585.pdf
dcterms.rightsHolderGeorg Thieme Verlag Kg
unesp.author.lattes3814504901386844[8]
unesp.author.orcid0000-0002-8645-3777[8]
unesp.author.orcid0000-0003-3032-2556[9]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt

Arquivos

Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Artigos - Química Orgânica - IQ
Artigos - Fisiologia - IBB