Response surface optimization of D(-)-lactic acid production by Lactobacillus SMI8 using corn steep liquor and yeast autolysate as an alternative nitrogen source

Bolner de Lima, Cristian J. [UNESP]; Coelho, Luciana Fontes [UNESP]; Blanco, Kate Cristina [UNESP]; Contiero, Jonas [UNESP]

Response surface optimization of D(-)-lactic acid production by Lactobacillus SMI8 using corn steep liquor and yeast autolysate as an alternative nitrogen source

Arquivos

WOS000273718700031.pdf (209.45 KB)

Data

2009-11-02

Autores

Bolner de Lima, Cristian J.

Coelho, Luciana Fontes

Blanco, Kate Cristina

Contiero, Jonas

Editor

Academic Journals

Tipo

Artigo

Direito de acesso

Acesso aberto

Resumo

The production of D(-) lactic acid from Lactobacillus LMI8 sp. was studied, using 2 low-cost nitrogen sources: corn steep liquor (CSL) and yeast autolysate (YA). A central composite design was used to determine maximal lactic acid production. The surface response method was used to evaluate the results of the experiments. The assays were performed in 250 mL Erlenmeyer flasks containing 100 mL of production medium maintained under refrigerated incubation at 200 rpm and a temperature of 37 +/- 1 degrees C for 48 h of fermentation. Lactic acid production was significantly affected by the isolated CSL as well as the interaction between CSL and YA. Maximal production of D(+) lactic acid was 41.42 g/L - a value located at the central point, which corresponded to 15 g/L of CSL and 5 g/L of YA.