Development of a comprehensive method for analyzing clerodane-type diterpenes and phenolic compounds from Casearia sylvestris Swartz (Salicaceae) based on ultra high performance liquid chromatography combined with chemometric tools

dc.contributor.authorPires Bueno, Paula Carolina [UNESP]
dc.contributor.authorVerbi Pereira, Fabiola Manhas [UNESP]
dc.contributor.authorTorres, Roseli Buzanelli
dc.contributor.authorCavalheiro, Alberto Jose [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionHerbarium IAC
dc.date.accessioned2015-10-21T20:24:56Z
dc.date.available2015-10-21T20:24:56Z
dc.date.issued2015-05-01
dc.description.abstractThis work describes the development and optimization of an analytical method utilizing liquid chromatography and chemometrics to evaluate and differentiate two varieties of Casearia sylvestris Swartz (Salicaceae) from São Paulo State (Brazil) based on their secondary metabolite profiles. Previously, analytical studies only concerned the analysis of clerodane-type diterpenes. Therefore, considering the importance of including phenolic compounds in such analysis, we used design of experiments to simultaneously extract and detect the largest number of compounds from both chemical classes. This new strategy allowed a comprehensive chromatographic analysis of C. sylvestris, and the results for the two varieties exhibited an interesting distribution according to their original ecosystems, suggesting a strong correlation to the main metabolites found in each species group. Besides their inherent morphological differences, C. sylvestris variety lingua, mainly found in Cerrado areas, predominantly contains phenolic compounds, while C. sylvestris variety sylvestris, mainly found in Atlantic Forest areas, contains mostly clerodane-type diterpenes. Finally, it was also possible to observe differences in the secondary metabolite composition within each group depending on the place where samples were collected.en
dc.description.affiliationUniversidade Estadual Paulista (UNESP), Instituto de Química (IQ), Departamento de Química Orgânica, BR-14800060 Araraquara, SP, Brasil
dc.description.affiliationUniversidade Estadual Paulista (UNESP), Instituto de Química (IQ), Departamento de Química Analítica, BR-14800060 Araraquara, SP, Brasil
dc.description.affiliationHerbarium IAC, Instituto Agronômico de Campinas, Campinas, SP, Brasil
dc.description.affiliationUnespUniversidade Estadual Paulista (UNESP), Instituto de Química (IQ), Departamento de Química Orgânica, BR-14800060 Araraquara, SP, Brasil
dc.description.affiliationUnespUniversidade Estadual Paulista (UNESP), Instituto de Química (IQ), Departamento de Química Analítica, BR-14800060 Araraquara, SP, Brasil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação para o Desenvolvimento da UNESP (FUNDUNESP)
dc.format.extent1649-1656
dc.identifierhttp://onlinelibrary.wiley.com/doi/10.1002/jssc.201401421/abstract
dc.identifier.citationJournal Of Separation Science. Weinheim: Wiley-v C H Verlag Gmbh, v. 38, n. 10, p. 1649-1656, 2015.
dc.identifier.doi10.1002/jssc.201401421
dc.identifier.issn1615-9306
dc.identifier.lattes2518006820764120
dc.identifier.urihttp://hdl.handle.net/11449/129125
dc.identifier.wosWOS:000355233100004
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofJournal Of Separation Science
dc.relation.ispartofjcr2.415
dc.relation.ispartofsjr0,795
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectCasearia sylvestrisen
dc.subjectChemical variabilityen
dc.subjectChemometricsen
dc.subjectMetabolitesen
dc.titleDevelopment of a comprehensive method for analyzing clerodane-type diterpenes and phenolic compounds from Casearia sylvestris Swartz (Salicaceae) based on ultra high performance liquid chromatography combined with chemometric toolsen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-Blackwell
unesp.author.lattes2518006820764120
unesp.author.lattes2518006820764120[4]
unesp.author.orcid0000-0001-8214-9957[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt

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