Ultrastructure of spermatophores and spermatozoa of intertidal crabs Pachygrapsus transversus, Pachygrapsus gracilis and Geograpsus lividus (Decapoda: Grapsidae)

Página do item simplificado
dc.contributor.authorTiseo, Gisele Rodrigues [UNESP]
dc.contributor.authorMantelatto, Fernando Luis
dc.contributor.authorZara, Fernando José [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2018-12-11T17:15:13Z
dc.date.available2018-12-11T17:15:13Z
dc.date.issued2017-07-01
dc.description.abstractPachygrapsus transversus, P. gracilis, and Geograpsus lividus are intertidal crabs, whose ultrastructure of spermatophores and spermatozoa are unknown. In this work, we characterized the ultrastructure of the spermatophores and spermatozoa of these three species comparing them to the results of previous research on molecular phylogeny of Grapsidae. Fragments of the posterior vas deferens were processed for routine in transmission electron microscopy. Pachygrapsus transversus and G. lividus exhibit coenospermic spermatophores, while Pachygrapsus gracilis presents cleistospermic spermatophores. The spermatozoa of all three species present the typical pattern seen in Thoracotremata. The acrosome is wider than long, with an acrosome length to width ratio of 0.82 ± 0.022 in P. transversus, 0.75 ± 0.008 in P. gracilis, and 0.82 ± 0.024 in G. lividus. The main specific sperm differences were observed in the perforatorial chamber, the operculum and the concentric acrosomal layers. Pachygrapsus transversus has a flask-shaped perforatorial chamber with a prominent narrowing under the apex, while Pachygrapsus gracilis exhibits a flask-shaped perforatorial chamber with a slight narrowing under the apex, thus wider than that of P. transversus. Geograpsus lividus presents a club-shaped perforatorial chamber with a conical apex. The acrosome of P. transversus has a narrow inner layer while the outer layer presents peripheral concentric lamellae near the opercular pole. In P. gracilis, the inner acrosomal layer of the acrosome is shifted toward the apical portion of the spermatozoon and the outer layer exhibits peripheral concentric lamellae throughout its extension. Geograpsus lividus shows a narrow and electron-dense inner layer shifted toward the opercular pole and curved operculum, unlike the pattern found for the Pachygrapsus species. The differences in the morphology of spermatophores suggest different strategies of transfer and/or storage of spermatozoa between the studied species. The spermatozoal ultrastructure supports the phylogenetic proximity of Pachygrapsus species and also demonstrate that spermiotaxonomy is a useful tool to be associated to molecular data.en
dc.description.affiliationDepartamento de Biologia Aplicada Laboratório de Morfologia do Invertebrados (IML) Centro de Aquicultura (CAUNESP) and IEAMar Univ. Estadual Paulista (UNESP), Prof. Donato Castellane, S/N, Jaboticabal
dc.description.affiliationLaboratório de Bioecologia e Sistemática de Crustáceos (LBSC) Departamento de Biologia Faculdade de Filosofia Ciências e Letras de Ribeirão Preto (FFCLRP) Universidade de São Paulo (USP), Av. Bandeirantes, 3900, Ribeirão Preto
dc.description.affiliationDepartamento de Zoologia Instituto de Biociências Universidade de São Paulo Cidade Universitária, Rua do Matão, travessa. 14, n. 101
dc.description.affiliationUnespDepartamento de Biologia Aplicada Laboratório de Morfologia do Invertebrados (IML) Centro de Aquicultura (CAUNESP) and IEAMar Univ. Estadual Paulista (UNESP), Prof. Donato Castellane, S/N, Jaboticabal
dc.format.extent166-176
dc.identifierhttp://dx.doi.org/10.1016/j.jcz.2017.07.002
dc.identifier.citationZoologischer Anzeiger, v. 269, p. 166-176.
dc.identifier.doi10.1016/j.jcz.2017.07.002
dc.identifier.file2-s2.0-85030698633.pdf
dc.identifier.issn0044-5231
dc.identifier.scopus2-s2.0-85030698633
dc.identifier.urihttp://hdl.handle.net/11449/175305
dc.language.isoeng
dc.relation.ispartofZoologischer Anzeiger
dc.relation.ispartofsjr0,623
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBrachyura
dc.subjectCleistospermy
dc.subjectCoenospermy
dc.subjectDIC
dc.subjectSpermiotaxonomy
dc.subjectTEM
dc.titleUltrastructure of spermatophores and spermatozoa of intertidal crabs Pachygrapsus transversus, Pachygrapsus gracilis and Geograpsus lividus (Decapoda: Grapsidae)en
dc.typeArtigo

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
2-s2.0-85030698633.pdf
Tamanho:
3.83 MB
Formato:
Adobe Portable Document Format
Descrição:
Baixar

Coleções

Artigos - Biologia - FCAV