An in vitro growth inhibition test for measuring the potency of Leptospira spp. Sejroe group vaccine in buffaloes

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dc.contributor.authorde Nardi Junior, Geraldo
dc.contributor.authorGenovez, Margareth Elide
dc.contributor.authorRibeiro, Márcio Garcia [UNESP]
dc.contributor.authorCastro, Vanessa
dc.contributor.authorJorge, Andre Mendes [UNESP]
dc.contributor.institutionCentro Pesquisa & Desenvolvimento Sanidade Anim
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:40:01Z
dc.date.available2014-05-20T13:40:01Z
dc.date.issued2010-07-01
dc.description.abstractLeptospira spp. serovars Hardjo and Wollfi from Sejroe serogroup have been detected in livestock in Brazil, where the main control procedures rely on vaccination. The potency of two commercial vaccines available in this country was monitored by microagglutination test-MAT and in vitro growth inhibition test-GIT in serum samples from 33 female buffaloes divided into: G1-unvaccinated control; G2-vaccinated with Leptobac-6 (R) containing serovars Hardjo and Wolffi and G3-vaccinated with Triangle-9 (R) containing serovar Hardjo. G2 and G3 animals were vaccinated on day zero, and received a booster and two revaccinations on days 30, 210 and 390 and G1 animals received phosphate buffered saline. Serum samples were collected at 15-day intervals between days 0 and 60; and at 30-day intervals between days 60 and 540 and were tested by MAT and GIT with serovars Hardjo and Wolffi. G1 remained negative throughout the experiment. Both vaccines were able to induce agglutinating and growth inhibition antibodies. Six months after the last revaccination, all animals tested negative by MAT, but still were positive by GIT until the end of experimental period. GIT could be a good tool to evaluate the potency and to monitor antibodies responses of vaccines of Sejroe group serovars. (C) 2010 The International Association for Biologicals. Published by Elsevier Ltd. All rights reserved.en
dc.description.affiliationCentro Pesquisa & Desenvolvimento Sanidade Anim, Inst Biol, BR-04014002 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Posgrad Fac Med Vet & Zootecnia, BR-05508 São Paulo, SP, Brazil
dc.description.affiliationFac Med Vet & Zootecnia UNESP Botucatu, São Paulo, Brazil
dc.description.affiliationUnespFac Med Vet & Zootecnia UNESP Botucatu, São Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent474-478
dc.identifierhttp://dx.doi.org/10.1016/j.biologicals.2010.02.014
dc.identifier.citationBiologicals. London: Academic Press Ltd- Elsevier B.V. Ltd, v. 38, n. 4, p. 474-478, 2010.
dc.identifier.doi10.1016/j.biologicals.2010.02.014
dc.identifier.issn1045-1056
dc.identifier.lattes2209124317273797
dc.identifier.lattes8878595413452908
dc.identifier.urihttp://hdl.handle.net/11449/13901
dc.identifier.wosWOS:000281190300008
dc.language.isoeng
dc.publisherAcademic Press Ltd Elsevier B.V. Ltd
dc.relation.ispartofBiologicals
dc.relation.ispartofjcr1.872
dc.relation.ispartofsjr0,636
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectBuffaloen
dc.subjectLeptospira spp. serovar Hardjoen
dc.subjectLeptospira spp. serovar Wolffien
dc.subjectLeptospirosis vaccineen
dc.subjectLeptospira growth inhibition testen
dc.titleAn in vitro growth inhibition test for measuring the potency of Leptospira spp. Sejroe group vaccine in buffaloesen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderAcademic Press Ltd- Elsevier B.V. Ltd
unesp.author.lattes2209124317273797
unesp.author.lattes8878595413452908[5]
unesp.author.orcid0000-0002-9079-198X[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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