Chemically Assisted Enucleation Results in Higher G6PD Expression in Early Bovine Female Embryos Obtained by Somatic Cell Nuclear Transfer

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dc.contributor.authorSaraiva, Naiara Zoccal [UNESP]
dc.contributor.authorOliveira, Clara Slade [UNESP]
dc.contributor.authorDrummond Tetzner, Tatiane Almeida [UNESP]
dc.contributor.authorde Lima, Marina Ragagnin [UNESP]
dc.contributor.authorde Melo, Danilas Salinet [UNESP]
dc.contributor.authorMeo Niciura, Simone Cristina
dc.contributor.authorGarcia, Joaquim Mansano [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.date.accessioned2014-05-20T13:15:23Z
dc.date.available2014-05-20T13:15:23Z
dc.date.issued2012-10-01
dc.description.abstractDespite extensive efforts, low efficiency is still an issue in bovine somatic cell nuclear transfer (SCNT). The hypothesis of our study was that the use of cytoplasts produced by chemically assisted enucleation (EN) would improve nuclear reprogramming in nuclear transfer (NT)-derived embryos because it results in lower damage and higher cytoplasm content than conventional EN. For that purpose, we investigated the expression of two X-linked genes: X inactive-specific transcript (XIST) and glucose 6-phosphate dehydrogenase (G6PD). In the first experiment, gene expression was assessed in day-7 female blastocysts from embryonic cell NT (ECNT) groups [conventional, ECNT conv; chemically assisted, ECNT deme (demecolcine)]. Whereas in the ECNT conv group, only one embryo (25%; n = 4) expressed XIST transcripts, most embryos showed XIST expression (75%; n = 4) in the ECNT deme group. However, no significant differences in transcript abundance of XIST and G6PD were found when comparing the embryos from all groups. In a second experiment using somatic cells as nuclear donors, we evaluated gene expression profiles in female SCNT-derived embryos. No significant differences in relative abundance (RA) of XIST transcripts were observed among the groups. Nonetheless, higher (p < 0.05) levels of G6PD were observed in SCNT deme and in vitro-derived groups in comparison to SCNT conv. To know whether higher G6PD expression in embryos derived from SCNT chemically assisted EN indicates higher metabolism in embryos considered of superior quality or if the presence of higher reactive oxygen species (ROS) levels generated by the increased oxygen consumption triggers G6PD activation, the expression of genes related to stress response should be investigated in embryos produced by that technique.en
dc.description.affiliationUniv Estadual Paulista, Dept Med Vet Prevent & Reprod Anim, Jaboticabal, Brazil
dc.description.affiliationEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Dairy Cattle Res Ctr, Juiz de Fora, Brazil
dc.description.affiliationEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Cattle SE CPPSE, Sao Carlos, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Med Vet Prevent & Reprod Anim, Jaboticabal, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent425-435
dc.identifierhttp://dx.doi.org/10.1089/cell.2011.0077
dc.identifier.citationCellular Reprogramming. New Rochelle: Mary Ann Liebert Inc., v. 14, n. 5, p. 425-435, 2012.
dc.identifier.doi10.1089/cell.2011.0077
dc.identifier.fileWOS000309545700006.pdf
dc.identifier.issn2152-4971
dc.identifier.lattes2251116139872527
dc.identifier.urihttp://hdl.handle.net/11449/2548
dc.identifier.wosWOS:000309545700006
dc.language.isoeng
dc.publisherMary Ann Liebert, Inc.
dc.relation.ispartofCellular Reprogramming
dc.relation.ispartofjcr1.430
dc.relation.ispartofsjr0,549
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleChemically Assisted Enucleation Results in Higher G6PD Expression in Early Bovine Female Embryos Obtained by Somatic Cell Nuclear Transferen
dc.typeArtigo
dcterms.licensehttp://www.liebertpub.com/archpolicy/journal-of-womens-health/42/
dcterms.rightsHolderMary Ann Liebert Inc.
unesp.author.lattes2251116139872527
unesp.author.orcid0000-0003-0046-0050[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt
unesp.departmentMedicina Veterinária Preventiva e Reprodução Animal - FCAVpt

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Jaboticabal - FCAV - Faculdade de Ciências Agrárias e Veterinárias