Collection and in vitro maturation of Mazama gouazoubira (brown brocket deer) oocytes obtained after ovarian stimulation

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dc.contributor.authorRola, Luciana Diniz [UNESP]
dc.contributor.authorZanetti, Eveline Dos Santos [UNESP]
dc.contributor.authorDel Collado, Maite
dc.contributor.authorPeroni, Ellen De Fátima Carvalho [UNESP]
dc.contributor.authorDuarte, José Maurício Barbanti [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Federal da Paraíba (UFPB)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2022-04-29T08:29:08Z
dc.date.available2022-04-29T08:29:08Z
dc.date.issued2020-01-01
dc.description.abstractSummary In vitro production of embryos has gained prominence as a tool for use in wildlife conservation programmes in situ and ex situ. However, the development of this technique depends on steps that include ovarian stimulation, collection and oocyte maturation. The purpose of this study was to assess the feasibility of an ovarian stimulation protocol for follicular aspiration, the efficiency of videolaparoscopy for follicular aspiration and test a medium for in vitro oocyte maturation for the species Mazama gouazoubira. Five females were submitted to repeated ovarian stimulation (hormone protocol using controlled internal drug release), and estradiol benzoate on D0 and eight injections of follicle-stimulating hormone, once every 12 h, from D4 onwards at 30-day intervals. Fourteen surgical procedures were performed in superstimulated females, resulting in the collection of 94 oocytes and an average of 17.1 ± 9.1 follicles observed, 13.5 ± 6.6 follicles aspirated and 7.2 ± 3.7 oocytes collected per surgery. After collection, the oocytes were submitted to in vitro maturation for 24 h and stained with Hoechst 33342 dye to evaluate their nuclear status; 64.5% of the oocytes reached MII and 16.1% were spontaneously activated by parthenogenesis. The nuclear status of oocytes that did not undergo in vitro maturation was evaluated; 80.9% were found to be immature.en
dc.description.affiliationNUPECCe - Núcleo de Pesquisa e Conservação de Cervídeos Departamento de Zootecnia Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista
dc.description.affiliationDepartamento de Zootecnia Centro de Ciências Agrárias Universidade Federal da Paraíba
dc.description.affiliationDepartamento de Medicina Veterinária Faculdade de Zootecnia e Engenharia de Alimentos Universidade de São Paulo
dc.description.affiliationUnespNUPECCe - Núcleo de Pesquisa e Conservação de Cervídeos Departamento de Zootecnia Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista
dc.identifierhttp://dx.doi.org/10.1017/S0967199420000787
dc.identifier.citationZygote.
dc.identifier.doi10.1017/S0967199420000787
dc.identifier.issn1469-8730
dc.identifier.issn0967-1994
dc.identifier.scopus2-s2.0-85099532306
dc.identifier.urihttp://hdl.handle.net/11449/228893
dc.language.isoeng
dc.relation.ispartofZygote
dc.sourceScopus
dc.subjectAssisted reproductive technologies
dc.subjectDeer
dc.subjectFSH
dc.subjectKeywords:
dc.subjectLaparoscopic ovum pick-up
dc.subjectWild animals
dc.titleCollection and in vitro maturation of Mazama gouazoubira (brown brocket deer) oocytes obtained after ovarian stimulationen
dc.typeArtigo
unesp.author.orcid0000-0003-3652-1011 0000-0003-3652-1011[1]

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