Chromosomal mapping of repetitive DNAs in the beetle Dichotomius geminatus provides the first evidence for an association of 5S rRNA and histone H3 genes in insects, and repetitive DNA similarity between the B chromosome and A complement

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dc.contributor.authorCabral-de-Mello, Diogo Cavalcanti [UNESP]
dc.contributor.authorMoura, R. C.
dc.contributor.authorMartins, C. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de Pernambuco (UFPE)
dc.date.accessioned2014-05-20T13:55:36Z
dc.date.available2014-05-20T13:55:36Z
dc.date.issued2010-04-01
dc.description.abstractChromosomal banding techniques and repetitive DNA mapping are useful tools in comparative analysis and in the elucidation of genome organization of several groups of eukaryotes. In this study, we contributed to the knowledge of Coleoptera genomes by reporting the chromosomal organization of repetitive DNA sequences, as well as the presence and characteristics of a B chromosome in two natural populations of Dichotomius geminatus (Coleoptera; Scarabaeidae) using classical, chromosomal banding and molecular cytogenetic techniques. As in other coleopteran species, the heterochromatin was mainly concentrated in pericentromeric regions and the B chromosome was composed almost entirely of heterochromatin. Physical mapping using double fluorescent in situ hybridization was performed for the first time in Coleoptera; using DNA probes for 5S and 18S ribosomal RNA (rRNA) and histone H3 genes, we showed that ribosomal 18S rDNAs are located in chromosomes 3 and 4, whereas 5S rRNA and histone H3 genes are colocalized in chromosomal pair 2 and show an apparently interspersed organization. Moreover, these genes are not present in the B chromosome, suggesting that the B chromosome did not originate from chromosomal pairs 2, 3 or 4. on the other hand, mapping of the C(0)t-1 DNA fraction showed that the B chromosome is enriched in repetitive DNA elements, also present in the standard complement, indicating an intraspecific origin of this element in D. geminatus. These results will contribute to our understanding of genome organization and evolution of repetitive elements in Coleoptera and other insects regarding both A and B chromosomes. Heredity (2010) 104, 393-400; doi: 10.1038/hdy.2009.126; published online 16 September 2009en
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias, Dept Morfol, BR-18618000 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de Pernambuco (UFPE), UPE, Inst Ciencias Biol, Dept Biol, Recife, PE, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias, Dept Morfol, BR-18618000 São Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE)
dc.format.extent393-400
dc.identifierhttp://dx.doi.org/10.1038/hdy.2009.126
dc.identifier.citationHeredity. London: Nature Publishing Group, v. 104, n. 4, p. 393-400, 2010.
dc.identifier.doi10.1038/hdy.2009.126
dc.identifier.issn0018-067X
dc.identifier.lattes8858800699425352
dc.identifier.orcid0000-0003-3534-974X
dc.identifier.urihttp://hdl.handle.net/11449/19902
dc.identifier.wosWOS:000275898200010
dc.language.isoeng
dc.publisherNature Publishing Group
dc.relation.ispartofHeredity
dc.relation.ispartofjcr3.872
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectcytogeneticsen
dc.subjectC(0)t-1 DNAen
dc.subjectFISHen
dc.subjectgenome evolutionen
dc.subjectheterochromatinen
dc.subjectsupernumerary chromosomeen
dc.titleChromosomal mapping of repetitive DNAs in the beetle Dichotomius geminatus provides the first evidence for an association of 5S rRNA and histone H3 genes in insects, and repetitive DNA similarity between the B chromosome and A complementen
dc.typeResumo
dcterms.licensehttp://www.nature.com/authors/author_resources/deposition.html
dcterms.rightsHolderNature Publishing Group
unesp.author.lattes8858800699425352[3]
unesp.author.orcid0000-0003-3534-974X[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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Artigos - Morfologia - IBB