Photodynamic inactivation of biofilms formed by Candida spp., Trichosporon mucoides, and Kodamaea ohmeri by cationic nanoemulsion of zinc 2,9,16,23-tetrakis(phenylthio)-29H, 31H-phthalocyanine (ZnPc)

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dc.contributor.authorJunqueira, Juliana Campos [UNESP]
dc.contributor.authorJorge, A. O. C. [UNESP]
dc.contributor.authorBarbosa, J. O. [UNESP]
dc.contributor.authorRossoni, R. D. [UNESP]
dc.contributor.authorVilela, S. F. G. [UNESP]
dc.contributor.authorCosta, A. C. B. P. [UNESP]
dc.contributor.authorPrimo, F. L.
dc.contributor.authorGoncalves, J. M.
dc.contributor.authorTedesco, A. C.
dc.contributor.authorSuleiman, J. M. A. H.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionInstituto de Infectologia Emílio Ribas
dc.date.accessioned2014-05-20T14:04:27Z
dc.date.available2014-05-20T14:04:27Z
dc.date.issued2012-11-01
dc.description.abstractThe biofilms formed by opportunistic yeasts serve as a persistent reservoir of infection and impair the treatment of fungal diseases. The aim of this study was to evaluate photodynamic inactivation (PDI) of biofilms formed by Candida spp. and the emerging pathogens Trichosporon mucoides and Kodamaea ohmeri by a cationic nanoemulsion of zinc 2,9,16,23-tetrakis(phenylthio)-29H,31H-phthalocyanine (ZnPc). Biofilms formed by yeasts after 48 h in the bottom of 96-well microtiter plates were treated with the photosensitizer (ZnPc) and a GaAlAs laser (26.3 J cm(-2)). The biofilm cells were scraped off the well wall, homogenized, and seeded onto Sabouraud dextrose agar plates that were then incubated at 37A degrees C for 48 h. Efficient PDI of biofilms was verified by counting colony-forming units (CFU/ml), and the data were submitted to analysis of variance and the Tukey test (p < 0.05). All biofilms studied were susceptible to PDI with statistically significant differences. The strains of Candida genus were more resistant to PDI than emerging pathogens T. mucoides and K. ohmeri. A mean reduction of 0.45 log was achieved for Candida spp. biofilms, and a reduction of 0.85 and 0.84, were achieved for biofilms formed by T. mucoides and K. ohmeri, respectively. Therefore, PDI by treatment with nanostructured formulations cationic zinc 2,9,16,23- tetrakis (phenylthio)- 29H, 31H- phthalocyanine (ZnPc) and a laser reduced the number of cells in the biofilms formed by strains of C. albicans and non-Candida albicans as well the emerging pathogens T. mucoides and K. ohmeri.en
dc.description.affiliationUNESP Univ Estadual Paulista, Dept Biosci & Oral Diag, Sch Dent Sao Jose dos Campos, BR-12245000 Sao Jose Dos Campos 12245000, SP, Brazil
dc.description.affiliationUniv São Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Lab Fotobiol & Fotomed,Dept Quim, Ctr Nanotecnol & Engn Tecidual Ribeirao Preto, BR-14040901 Ribeirao Preto, SP, Brazil
dc.description.affiliationEmilio Ribas Inst Infectol, BR-01246900 São Paulo, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Dept Biosci & Oral Diag, Sch Dent Sao Jose dos Campos, BR-12245000 Sao Jose Dos Campos 12245000, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipPró-Reitoria de Pesquisa da UNESP (PROPe UNESP)
dc.description.sponsorshipIdFAPESP: 09/52283-0
dc.description.sponsorshipIdFAPESP: 09/15363-6
dc.format.extent1205-1212
dc.identifierhttp://dx.doi.org/10.1007/s10103-012-1050-2
dc.identifier.citationLasers In Medical Science. London: Springer London Ltd, v. 27, n. 6, p. 1205-1212, 2012.
dc.identifier.doi10.1007/s10103-012-1050-2
dc.identifier.issn0268-8921
dc.identifier.lattes0322020541055900
dc.identifier.lattes0053567153623569
dc.identifier.lattes1664688357804299
dc.identifier.urihttp://hdl.handle.net/11449/22612
dc.identifier.wosWOS:000309346600011
dc.language.isoeng
dc.publisherSpringer London Ltd
dc.relation.ispartofLasers in Medical Science
dc.relation.ispartofjcr1.949
dc.relation.ispartofsjr0,713
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectBiofilmen
dc.subjectCandida spp.en
dc.subjectTrichosporon mucoidesen
dc.subjectKodamaea ohmerien
dc.subjectPhotodynamic inactivationen
dc.subjectZinc phthalocyanineen
dc.titlePhotodynamic inactivation of biofilms formed by Candida spp., Trichosporon mucoides, and Kodamaea ohmeri by cationic nanoemulsion of zinc 2,9,16,23-tetrakis(phenylthio)-29H, 31H-phthalocyanine (ZnPc)en
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer London Ltd
unesp.author.lattes0322020541055900
unesp.author.lattes0053567153623569[2]
unesp.author.lattes1664688357804299
unesp.author.orcid0000-0002-1747-6158[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Ciência e Tecnologia, São José dos Campospt

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