Oxidative Stress Alters the Profile of Transcription Factors Related to Early Development on in Vitro Produced Embryos

Página do item simplificado
dc.contributor.authorLeite, Roberta Ferreira
dc.contributor.authorAnnes, Kelly
dc.contributor.authorIspada, Jessica
dc.contributor.authorDe Lima, Camila Bruna
dc.contributor.authorDos Santos, Érika Cristina
dc.contributor.authorFontes, Patricia Kubo [UNESP]
dc.contributor.authorNogueira, Marcelo Fábio Gouveia [UNESP]
dc.contributor.authorMilazzotto, Marcella Pecora
dc.contributor.institutionUniversidade Federal do ABC (UFABC)
dc.contributor.institutionSciences Universidade
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:18:17Z
dc.date.available2018-12-11T17:18:17Z
dc.date.issued2017-01-01
dc.description.abstractHigh oxygen levels during in vitro culture (IVC) can induce oxidative stress through accumulation of reactive oxygen species (ROS), negatively affecting embryo development. This study evaluated the effect of different O 2 tensions during IVC on bovine blastocyst development and transcriptional status, considering transcription factors that play an essential role during early embryo development. For this purpose, embryos were produced in vitro by conventional protocols and cultured in two different oxygen tensions, physiological (5%) and atmospheric (20%). Expanded blastocysts were subjected to transcript quantitation analysis by RT-qPCR with Biomark™ HD System (Fluidigm, US), using 67 TaqMan assays specific for Bos taurus. Differences were observed in genes related to oxidation-reduction processes, DNA-dependent transcription factors, and factors related to important functional pathways for embryo development. Blastocyst rate was higher in the 5% O 2 group and the number of cells was assessed, with the 5% O 2 group having a higher number of cells. ROS concentration was evaluated, with a higher ROS presence in the 20% O 2 group. Taken together, these results allow us to conclude that IVC of embryos at atmospheric O 2 tension affects the expression of important transcription factors involved in multiple cell biology pathways that can affect embryo development, quality, and viability.en
dc.description.affiliationCenter of Natural and Human Sciences Universidade Federal Do ABC
dc.description.affiliationInstitute of Biomedical Sciences Universidade
dc.description.affiliationInstitute of Biosciences Campus Botucatu Department of Pharmacology Universidade Estadual Paulista (UNESP)
dc.description.affiliationSchool of Sciences and Languages Campus Assis Department of Biological Sciences Universidade Estadual Paulista (UNESP)
dc.description.affiliationUnespInstitute of Biosciences Campus Botucatu Department of Pharmacology Universidade Estadual Paulista (UNESP)
dc.description.affiliationUnespSchool of Sciences and Languages Campus Assis Department of Biological Sciences Universidade Estadual Paulista (UNESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.identifierhttp://dx.doi.org/10.1155/2017/1502489
dc.identifier.citationOxidative Medicine and Cellular Longevity, v. 2017.
dc.identifier.doi10.1155/2017/1502489
dc.identifier.file2-s2.0-85042624694.pdf
dc.identifier.issn1942-0994
dc.identifier.issn1942-0900
dc.identifier.lattes3734933152414412
dc.identifier.scopus2-s2.0-85042624694
dc.identifier.urihttp://hdl.handle.net/11449/175953
dc.language.isoeng
dc.relation.ispartofOxidative Medicine and Cellular Longevity
dc.relation.ispartofsjr1,558
dc.relation.ispartofsjr1,558
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.titleOxidative Stress Alters the Profile of Transcription Factors Related to Early Development on in Vitro Produced Embryosen
dc.typeArtigo
unesp.author.lattes3734933152414412
unesp.author.orcid0000-0003-0933-3066[8]
unesp.departmentCiências Biológicas - FCLASpt

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
2-s2.0-85042624694.pdf
Tamanho:
9.1 MB
Formato:
Adobe Portable Document Format
Descrição:
Baixar

Coleções

Artigos - Farmacologia - IBB
Assis - FCLAS - Faculdade de Ciências e Letras