Acquisition and maintenance of pluripotency are influenced by fibroblast growth factor, leukemia inhibitory factor, and 2i in bovine-induced pluripotent stem cells

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dc.contributor.authorBotigelli, Ramon Cesar [UNESP]
dc.contributor.authorPieri, Naira Carolina Godoy
dc.contributor.authorBessi, Brendon William
dc.contributor.authorMachado, Lucas Simões
dc.contributor.authorBridi, Alessandra
dc.contributor.authorde Souza, Aline Fernanda
dc.contributor.authorRecchia, Kaiana
dc.contributor.authorNeto, Paulo Fantinato
dc.contributor.authorRoss, Pablo Juan
dc.contributor.authorBressan, Fabiana Fernandes
dc.contributor.authorNogueira, Marcelo Fábio Gouveia [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversity of Guelph
dc.contributor.institutionDavis
dc.date.accessioned2023-07-29T13:22:57Z
dc.date.available2023-07-29T13:22:57Z
dc.date.issued2022-09-14
dc.description.abstractSeveral opportunities for embryo development, stem cell maintenance, cell fate, and differentiation have emerged using induced pluripotent stem cells (iPSCs). However, the difficulty in comparing bovine iPSCs (biPSCs) with embryonic stem cells (ESCs) was a challenge for many years. Here, we reprogrammed fetal fibroblasts by transient expression of the four transcription factors (Oct4, Sox2, Klf4, and c-Myc, collectively termed “OSKM” factors) and cultured in iPSC medium, supplemented with bFGF, bFGF2i, leukemia inhibitory factor (LIF), or LIF2i, and then compared these biPSC lines with bESC to evaluate the pluripotent state. biPSC lines were generated in all experimental groups. Particularly, reprogrammed cells treated with bFGF were more efficient in promoting the acquisition of pluripotency. However, LIF2i treatment did not promote continuous self-renewal. biPSCs (line 2) labeled with GFP were injected into early embryos (day 4.5) to assess the potential to contribute to chimeric blastocysts. The biPSC lines show a pluripotency state and are differentiated into three embryonic layers. Moreover, biPSCs and bESCs labeled with GFP were able to contribute to chimeric blastocysts. Additionally, biPSCs have shown promising potential for contributing to chimeric blastocysts and for future studies.en
dc.description.affiliationMultiuser Facility (FitoFarmaTec) Department of Pharmacology Biosciences Institute (IBB) São Paulo State University (UNESP)
dc.description.affiliationLaboratory of Molecular Morphophysiology and Development (LMMD) Department of Veterinary Medicine Faculty of Animal Science and Food Engineering (FZEA) University of São Paulo (USP)
dc.description.affiliationPaulista School of Medicine (EPM) Laboratory of Neurobiology Department of Biochemistry Federal University of São Paulo (UNIFESP)
dc.description.affiliationLaboratory Biomedical Science Department of Biomedical Science Ontario Veterinary College (OVC) University of Guelph
dc.description.affiliationLaboratory De partment of Animal Science University of California Davis
dc.description.affiliationSchool of Sciences and Languages Laboratory of Embryonic Micromanipulation Department of Biological Sciences São Paulo State University (UNESP)
dc.description.affiliationUnespMultiuser Facility (FitoFarmaTec) Department of Pharmacology Biosciences Institute (IBB) São Paulo State University (UNESP)
dc.description.affiliationUnespSchool of Sciences and Languages Laboratory of Embryonic Micromanipulation Department of Biological Sciences São Paulo State University (UNESP)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2012/50533-2 2015/26818-5 2016/16841-2 2013/08135-2 2019/10751-0 23038.006964/2014-43 433133/2018-0
dc.identifierhttp://dx.doi.org/10.3389/fcell.2022.938709
dc.identifier.citationFrontiers in Cell and Developmental Biology, v. 10.
dc.identifier.doi10.3389/fcell.2022.938709
dc.identifier.issn2296-634X
dc.identifier.scopus2-s2.0-85138967942
dc.identifier.urihttp://hdl.handle.net/11449/247683
dc.language.isoeng
dc.relation.ispartofFrontiers in Cell and Developmental Biology
dc.sourceScopus
dc.subjectbovine
dc.subjectcellular reprogramming
dc.subjectembryonic stem cells
dc.subjectinduced pluripotent stem cells
dc.subjectpluripotency maintenance
dc.titleAcquisition and maintenance of pluripotency are influenced by fibroblast growth factor, leukemia inhibitory factor, and 2i in bovine-induced pluripotent stem cellsen
dc.typeArtigo

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