PARADOXICAL EFFECT OF QUERCETIN ANTIOXIDANT ON GOAT SPERM PARAMETERS AFTER CRYOPRESERVATION

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dc.contributor.authorBorges, Marcelo Sant'Ana
dc.contributor.authorBarbosa Born, Joana Larissa
dc.contributor.authorConti, Leticia Martins
dc.contributor.authorSegabinazzi, Lorenzo Garrido [UNESP]
dc.contributor.authorNichi, Marcilio
dc.contributor.authorVechiato Kawai, Giulia Kiyomi
dc.contributor.authorLeite, Roberta Ferreira
dc.contributor.authorPeixoto, Kleber da Cunha
dc.contributor.authorDell'Aqua, Jose Antonio [UNESP]
dc.contributor.authorPapa, Frederico Ozanam [UNESP]
dc.contributor.authorCrespilho, Andre Maciel
dc.contributor.institutionUniv Santo Amaro
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2020-12-10T20:14:02Z
dc.date.available2020-12-10T20:14:02Z
dc.date.issued2020-05-01
dc.description.abstractBACKGROUND: Some antioxidants have been used in semen extenders to reduce adverse effects caused by excessive reactive oxygen species (ROS) production. The study was carried out to assess the effect of quercetin (QC) antioxidant therapy on goat semen submitted to cryopreservation. OBJECTIVE: To evaluate the effect of quercetin incorporation in different phases of the cryopreservation process of goat spermatozoa. MATERIALS AND METHODS: Five ejaculates from each of four goats (n=20) were collected and split into four groups: Control (G1), without QC; G2, 15 mu M of QC added to semen before centrifugation; G3, 15 mu M QC added to semen after centrifugation; G4, 15 mu M QC added to semen before centrifugation and 15 mu M of QC added to semen after centrifugation (total of 30 mu M of QC); and cryopreserved. All semen samples were evaluated after thawing for sperm kinetics, plasma membrane integrity, and ROS levels. RESULTS: Although lower concentrations of ROS were associated with groups that received antioxidant supplementation (P=0.0213), linear and dose dependent (P<0.05) reductions of the total and progressive sperm motility, velocity and percentage of fast cells were related to the QC groups. Likewise, plasma membrane integrity was better preserved (P=0.0154) in the control group (35.5%) than in groups that received QC (G2=32.6%, G3=32.4% and G4=26.7%). CONCLUSION: Although quercetin was efficient at reducing the oxidative stress related to sperm cryopreservation, it exerted a deleterious dose-dependent effect on the kinetics and integrity of the frozen goat semen, contradicting its use at the tested concentrations.en
dc.description.affiliationUniv Santo Amaro, UNISA, Sao Paulo, SP, Brazil
dc.description.affiliationSao Paulo State Univ UNESP, Sch Vet Med & Anim Sci, Botucatu, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Fac Vet Med & Anim Sci FMVZ, Dept Anim Reprod VRA, Sao Paulo, Brazil
dc.description.affiliationUnespSao Paulo State Univ UNESP, Sch Vet Med & Anim Sci, Botucatu, SP, Brazil
dc.format.extent128-134
dc.identifier.citationCryoletters. London: Cryo Letters, v. 41, n. 3, p. 128-134, 2020.
dc.identifier.issn0143-2044
dc.identifier.urihttp://hdl.handle.net/11449/197341
dc.identifier.wosWOS:000534630200002
dc.language.isoeng
dc.publisherCryo Letters
dc.relation.ispartofCryoletters
dc.sourceWeb of Science
dc.subjectantioxidant
dc.subjectcryopreservation
dc.subjectgoat
dc.subjectquercetin
dc.subjectsperm quality
dc.titlePARADOXICAL EFFECT OF QUERCETIN ANTIOXIDANT ON GOAT SPERM PARAMETERS AFTER CRYOPRESERVATIONen
dc.typeArtigo
dcterms.rightsHolderCryo Letters

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