Is the production of reactive oxygen and nitrogen species by macrophages associated with better infectious control in female mice with experimentally disseminated and pulmonary mucormycosis?

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dc.contributor.authordos Santos, Amanda Ribeiro
dc.contributor.authorFraga-Silva, Thais Fernanda
dc.contributor.authorde Fátima Almeida-Donanzam, Débora
dc.contributor.authorFinatto, Angela Carolina [UNESP]
dc.contributor.authorMarchetti, Camila [UNESP]
dc.contributor.authorAndrade, Maria Izilda
dc.contributor.authorde Arruda, Olavo Speranza [UNESP]
dc.contributor.authorde Arruda, Maria Sueli Parreira [UNESP]
dc.contributor.authorVenturini, James
dc.contributor.institutionUniversidade Federal de Mato Grosso do Sul (UFMS)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionLauro de Souza Lima Institute
dc.date.accessioned2023-07-29T14:00:07Z
dc.date.available2023-07-29T14:00:07Z
dc.date.issued2022-12-01
dc.description.abstractDifferent levels of resistance against Rhizopus oryzae infection have been observed between inbred (BALB/c) and outbred (Swiss) mice and are associated with the genetic background of each mouse strain. Considering that macrophages play an important role in host resistance to Rhizopus species, we used different infectious outcomes observed in experimental mucormycosis to identify the most efficient macrophage response pattern against R. oryzae in vitro and in vivo. For this, we compared BALB/c and Swiss macrophage activity before and after intravenous or intratracheal R. oryzae infections. The production of hydrogen peroxide (H2O2) and nitric oxide (NO) was determined in cultures of peritoneal (PMΦ) or alveolar macrophages (AMΦ) challenged with heat-killed spores of R. oryzae. The levels of tumor necrosis factor-alpha (TNF-α) and interleukin-10 (IL-10) were measured to confirm our findings. Naïve PMΦ from female BALB/c mice showed increased production of H2O2, TNF-α, and IL-10 in the presence of heat-killed spores of R. oryzae. Naïve PMΦ from female Swiss mice were less responsive. Naïve AMΦ from the two strains of female mice were less reactive to heat-killed spores of R. oryzae than PMΦ. After 30 days of R. oryzae intravenous infection, lower fungal load in spleen from BALB/c mice was accompanied by higher production of H2O2 by PMΦ compared with Swiss mice. In contrast, AMΦ from BALB/c mice showed higher production of NO, TNF-α, and IL-10 after 7 days of intratracheal infection. The collective findings reveal that, independent of the female mouse strain, PMΦ is more reactive against R. oryzae upon first contact than AMΦ. In addition, increased PMΦ production of H2O2 at the end of disseminated infection is accompanied by better fungal clearance in resistant (BALB/c) mice. Our findings further the understanding of the parasite–host relationship in mucormycosis.en
dc.description.affiliationFaculdade de Medicina Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande
dc.description.affiliationDepartamento de Bioquímica e Imunologia Escola de Medicina de Ribeirão Preto Universidade de São Paulo, São Paulo
dc.description.affiliationFaculdade de Ciências Universidade Estadual Paulista (Unesp), Bauru
dc.description.affiliationLauro de Souza Lima Institute, Bauru
dc.description.affiliationUnespFaculdade de Ciências Universidade Estadual Paulista (Unesp), Bauru
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2013/15513-3
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0270071
dc.identifier.citationPLoS ONE, v. 17, n. 12 December, 2022.
dc.identifier.doi10.1371/journal.pone.0270071
dc.identifier.issn1932-6203
dc.identifier.scopus2-s2.0-85144193097
dc.identifier.urihttp://hdl.handle.net/11449/249016
dc.language.isoeng
dc.relation.ispartofPLoS ONE
dc.sourceScopus
dc.titleIs the production of reactive oxygen and nitrogen species by macrophages associated with better infectious control in female mice with experimentally disseminated and pulmonary mucormycosis?en
dc.typeArtigo

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