Influence of apitoxin and melittin from Apis mellifera bee on Staphylococcus aureus strains

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dc.contributor.authorMarques Pereira, Ana Flavia [UNESP]
dc.contributor.authorAlbano, Mariana [UNESP]
dc.contributor.authorBergamo Alves, Fernanda Cristina [UNESP]
dc.contributor.authorMurbach Teles Andrade, Bruna Fernanda [UNESP]
dc.contributor.authorFurlanetto, Alessandra [UNESP]
dc.contributor.authorMores Rall, Vera Lucia [UNESP]
dc.contributor.authorSantos, Lucilene Delazari dos [UNESP]
dc.contributor.authorOrsi, Ricardo de Oliveira [UNESP]
dc.contributor.authorFernandes Junior, Ary [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2020-12-10T19:57:31Z
dc.date.available2020-12-10T19:57:31Z
dc.date.issued2020-04-01
dc.description.abstractThe antibacterial activities of apitoxin, a venom produced by Apis mellifera bee, and melittin, an antimicrobial peptide from apitoxin, were tested against planktonic and biofilm states of Staphylococcus aureus methicillin-resistant (MRSA), including clinical, and enterotoxin-producing isolates. Also, the synergism of apitoxin and melittin in combination with oxacillin were evaluated as well. The induced morphological changes on S. aureus cells of both products were detected by transmission electronic microscopy (TEM). The minimum inhibitory concentration (MIC) values were 7.2 mu g/mL, and 6.7 mu g/mL, for apitoxin and melittin, respectively. The minimum bactericidal concentration (MBC) values were 28.7 mu g/mL, and 26 mu g/mL for apitoxin and melittin, respectively. The time-kill curve assays of apitoxin or melittin with oxacillin exhibited bactericidal synergism against MRSA isolates. TEM images showed cell distortion, cell disintegration with leakage of cytoplasmic content and loss of cytoplasm content. However, apitoxin and melittin did not interfere with staphylococcal enterotoxin production or release. Thus, apitoxin and melittin are potential agents against MRSA that can serve as possible models for new antibacterial drugs.en
dc.description.affiliationSao Paulo State Univ, Dept Microbiol & Immunol, BR-18618691 Botucatu, SP, Brazil
dc.description.affiliationSao Paulo State Univ, CEVAP Ctr Study Venoms & Venomous Anim, BR-18610307 Botucatu, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Dept Microbiol & Immunol, BR-18618691 Botucatu, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ, CEVAP Ctr Study Venoms & Venomous Anim, BR-18610307 Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipElectronic Microscopy Center, Institute of Biosciences of Botucatu (IBB), Sao Paulo State University (UNESP), Botucatu, Brazil
dc.description.sponsorshipIdFAPESP: 2015/14278-6
dc.description.sponsorshipIdCNPq: 162208/2015-4
dc.format.extent8
dc.identifierhttp://dx.doi.org/10.1016/j.micpath.2020.104011
dc.identifier.citationMicrobial Pathogenesis. London: Academic Press Ltd- Elsevier Science Ltd, v. 141, 8 p., 2020.
dc.identifier.doi10.1016/j.micpath.2020.104011
dc.identifier.issn0882-4010
dc.identifier.urihttp://hdl.handle.net/11449/196829
dc.identifier.wosWOS:000528844800025
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofMicrobial Pathogenesis
dc.sourceWeb of Science
dc.subjectantibacterial activity
dc.subjectBee venom
dc.subjectAntimicrobial peptides
dc.subjectMethicillin-resistant Staphylococcus aureus
dc.subjectMRSA
dc.subjectEnterotoxin-producing S. aureus
dc.titleInfluence of apitoxin and melittin from Apis mellifera bee on Staphylococcus aureus strainsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes8310835825824360[9]
unesp.author.orcid0000-0002-2925-8545[1]
unesp.author.orcid0000-0001-8099-8277[8]
unesp.author.orcid0000-0002-2744-2824[9]

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Artigos - Microbiologia e Imunologia - IBB
Botucatu - CEVAP - Centro de Estudos de Venenos e Animais Peçonhentos