Caffeine and Chlorogenic Acid Combination Attenuate Early-Stage Chemically Induced Colon Carcinogenesis in Mice: Involvement of oncomiR miR-21a-5p

Página do item simplificado
dc.contributor.authorBartolomeu, Ariane Rocha [UNESP]
dc.contributor.authorRomualdo, Guilherme Ribeiro [UNESP]
dc.contributor.authorLisón, Carmen Griñán
dc.contributor.authorBesharat, Zein Mersini
dc.contributor.authorCorrales, Juan Antonio Marchal
dc.contributor.authorChaves, Maria Ángel García
dc.contributor.authorBarbisan, Luís Fernando [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversity of Granada
dc.contributor.institutionPfizer/University of Granada/Andalusian Regional Government
dc.contributor.institutionComplejo Hospitalario de Jaen
dc.contributor.institutionSapienza University of Rome
dc.date.accessioned2023-03-01T20:46:42Z
dc.date.available2023-03-01T20:46:42Z
dc.date.issued2022-06-01
dc.description.abstractColorectal cancer (CRC) is one of most common cancers worldwide, with high rates of mortality. Epidemiological findings demonstrate that coffee consumption reduces the risk of developing CRC by ~13%. In general, in vivo and in vitro findings demonstrate the antiproliferative, antioxidant and proapoptotic effects of brewed coffee or major bioavailable coffee compounds. Thus, it was assessed whether caffeine (CAF) and/or chlorogenic acid (CGA) attenuates the early-stage of chemically induced mouse colon carcinogenesis. Male Swiss mice were submitted to a 1,2-dimethylhydrazine/deoxycholic acid (DMH/DCA)-induced colon carcinogenesis model. These animals received CAF (50 mg/kg), CGA (25 mg/kg) or CAF+CGA (50 + 25 mg/kg) intragastrically for five times/week for ten weeks. CAF+CGA had the most pronounced effects on decreasing epithelial cell proliferation (Ki-67) and increasing apoptosis (cleaved caspase-3) in colonic crypts. This treatment also decreased the levels of proinflammatory cytokines IL-6, IL-17 and TNF-α, and downregulated the oncomiR miR-21a-5p in the colon. Accordingly, the analysis of miR-21a-5p targets demonstrated the genes involved in the negative regulation of proliferation and inflammation, and the positive regulation of apoptosis. Ultimately, CAF+CGA attenuated preneoplastic aberrant crypt foci (ACF) development. Our findings suggest that a combination of coffee compounds reduces early-stage colon carcinogenesis by the modulation of miR-21a-5p expression, highlighting the importance of coffee intake to prevent CRC.en
dc.description.affiliationDepartment of Pathology Botucatu Medical School São Paulo State University (UNESP), SP
dc.description.affiliationDepartment of Structural and Functional Biology Biosciences Institute São Paulo State University (UNESP), SP
dc.description.affiliationBiosanitary Research Institute of Granada (ibs.GRANADA) University Hospitals of Granada University of Granada
dc.description.affiliationGENYO (Centre for Genomics and Oncological Research) Pfizer/University of Granada/Andalusian Regional Government
dc.description.affiliationUGC de Oncología Médica Complejo Hospitalario de Jaen
dc.description.affiliationDepartment of Experimental Medicine Sapienza University of Rome
dc.description.affiliationDepartment of Human Anatomy and Embryology School of Medicine University of Granada
dc.description.affiliationDepartment of Biochemistry and Molecular Biology III and Immunology University of Granada
dc.description.affiliationUnespDepartment of Pathology Botucatu Medical School São Paulo State University (UNESP), SP
dc.description.affiliationUnespDepartment of Structural and Functional Biology Biosciences Institute São Paulo State University (UNESP), SP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: #2016/12015–0
dc.description.sponsorshipIdFAPESP: #2016/14420–0
dc.description.sponsorshipIdFAPESP: #2017/26217-7
dc.identifierhttp://dx.doi.org/10.3390/ijms23116292
dc.identifier.citationInternational Journal of Molecular Sciences, v. 23, n. 11, 2022.
dc.identifier.doi10.3390/ijms23116292
dc.identifier.issn1422-0067
dc.identifier.issn1661-6596
dc.identifier.scopus2-s2.0-85131179138
dc.identifier.urihttp://hdl.handle.net/11449/241091
dc.language.isoeng
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.sourceScopus
dc.subjectcaffeine
dc.subjectchemically induced colon carcinogenesis
dc.subjectchlorogenic acid
dc.subjectmice
dc.subjectmiRNA expression
dc.titleCaffeine and Chlorogenic Acid Combination Attenuate Early-Stage Chemically Induced Colon Carcinogenesis in Mice: Involvement of oncomiR miR-21a-5pen
dc.typeArtigo

Arquivos

Coleções

Artigos - Patologia - FMB