Caffeine and Chlorogenic Acid Combination Attenuate Early-Stage Chemically Induced Colon Carcinogenesis in Mice: Involvement of oncomiR miR-21a-5p
dc.contributor.author | Bartolomeu, Ariane Rocha [UNESP] | |
dc.contributor.author | Romualdo, Guilherme Ribeiro [UNESP] | |
dc.contributor.author | Lisón, Carmen Griñán | |
dc.contributor.author | Besharat, Zein Mersini | |
dc.contributor.author | Corrales, Juan Antonio Marchal | |
dc.contributor.author | Chaves, Maria Ángel García | |
dc.contributor.author | Barbisan, Luís Fernando [UNESP] | |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | |
dc.contributor.institution | University of Granada | |
dc.contributor.institution | Pfizer/University of Granada/Andalusian Regional Government | |
dc.contributor.institution | Complejo Hospitalario de Jaen | |
dc.contributor.institution | Sapienza University of Rome | |
dc.date.accessioned | 2023-03-01T20:46:42Z | |
dc.date.available | 2023-03-01T20:46:42Z | |
dc.date.issued | 2022-06-01 | |
dc.description.abstract | Colorectal cancer (CRC) is one of most common cancers worldwide, with high rates of mortality. Epidemiological findings demonstrate that coffee consumption reduces the risk of developing CRC by ~13%. In general, in vivo and in vitro findings demonstrate the antiproliferative, antioxidant and proapoptotic effects of brewed coffee or major bioavailable coffee compounds. Thus, it was assessed whether caffeine (CAF) and/or chlorogenic acid (CGA) attenuates the early-stage of chemically induced mouse colon carcinogenesis. Male Swiss mice were submitted to a 1,2-dimethylhydrazine/deoxycholic acid (DMH/DCA)-induced colon carcinogenesis model. These animals received CAF (50 mg/kg), CGA (25 mg/kg) or CAF+CGA (50 + 25 mg/kg) intragastrically for five times/week for ten weeks. CAF+CGA had the most pronounced effects on decreasing epithelial cell proliferation (Ki-67) and increasing apoptosis (cleaved caspase-3) in colonic crypts. This treatment also decreased the levels of proinflammatory cytokines IL-6, IL-17 and TNF-α, and downregulated the oncomiR miR-21a-5p in the colon. Accordingly, the analysis of miR-21a-5p targets demonstrated the genes involved in the negative regulation of proliferation and inflammation, and the positive regulation of apoptosis. Ultimately, CAF+CGA attenuated preneoplastic aberrant crypt foci (ACF) development. Our findings suggest that a combination of coffee compounds reduces early-stage colon carcinogenesis by the modulation of miR-21a-5p expression, highlighting the importance of coffee intake to prevent CRC. | en |
dc.description.affiliation | Department of Pathology Botucatu Medical School São Paulo State University (UNESP), SP | |
dc.description.affiliation | Department of Structural and Functional Biology Biosciences Institute São Paulo State University (UNESP), SP | |
dc.description.affiliation | Biosanitary Research Institute of Granada (ibs.GRANADA) University Hospitals of Granada University of Granada | |
dc.description.affiliation | GENYO (Centre for Genomics and Oncological Research) Pfizer/University of Granada/Andalusian Regional Government | |
dc.description.affiliation | UGC de Oncología Médica Complejo Hospitalario de Jaen | |
dc.description.affiliation | Department of Experimental Medicine Sapienza University of Rome | |
dc.description.affiliation | Department of Human Anatomy and Embryology School of Medicine University of Granada | |
dc.description.affiliation | Department of Biochemistry and Molecular Biology III and Immunology University of Granada | |
dc.description.affiliationUnesp | Department of Pathology Botucatu Medical School São Paulo State University (UNESP), SP | |
dc.description.affiliationUnesp | Department of Structural and Functional Biology Biosciences Institute São Paulo State University (UNESP), SP | |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
dc.description.sponsorshipId | FAPESP: #2016/12015–0 | |
dc.description.sponsorshipId | FAPESP: #2016/14420–0 | |
dc.description.sponsorshipId | FAPESP: #2017/26217-7 | |
dc.identifier | http://dx.doi.org/10.3390/ijms23116292 | |
dc.identifier.citation | International Journal of Molecular Sciences, v. 23, n. 11, 2022. | |
dc.identifier.doi | 10.3390/ijms23116292 | |
dc.identifier.issn | 1422-0067 | |
dc.identifier.issn | 1661-6596 | |
dc.identifier.scopus | 2-s2.0-85131179138 | |
dc.identifier.uri | http://hdl.handle.net/11449/241091 | |
dc.language.iso | eng | |
dc.relation.ispartof | International Journal of Molecular Sciences | |
dc.source | Scopus | |
dc.subject | caffeine | |
dc.subject | chemically induced colon carcinogenesis | |
dc.subject | chlorogenic acid | |
dc.subject | mice | |
dc.subject | miRNA expression | |
dc.title | Caffeine and Chlorogenic Acid Combination Attenuate Early-Stage Chemically Induced Colon Carcinogenesis in Mice: Involvement of oncomiR miR-21a-5p | en |
dc.type | Artigo |