Myocardial Dysfunction after Severe Food Restriction Is Linked to Changes in the Calcium-Handling Properties in Rats

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dc.contributor.authorDeus, Adriana Fernandes de [UNESP]
dc.contributor.authorSilva, Vitor Loureiro da [UNESP]
dc.contributor.authorBorges de Souza, Sergio Luiz [UNESP]
dc.contributor.authorFerreira Mota, Gustavo Augusto [UNESP]
dc.contributor.authorSant'Ana, Paula Grippa [UNESP]
dc.contributor.authorVileigas, Danielle Fernandes [UNESP]
dc.contributor.authorLima-Leopoldo, Ana Paula
dc.contributor.authorLeopoldo, Andre Soares
dc.contributor.authorSalome de Campos, Dijon Henrique [UNESP]
dc.contributor.authorTomasi, Loreta Casquel de [UNESP]
dc.contributor.authorPadovani, Carlos Roberto [UNESP]
dc.contributor.authorKolwicz, Stephen C.
dc.contributor.authorCicogna, Antonio Carlos [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal do Espírito Santo (UFES)
dc.contributor.institutionUrsinus Coll
dc.date.accessioned2020-12-10T19:36:52Z
dc.date.available2020-12-10T19:36:52Z
dc.date.issued2019-09-01
dc.description.abstractSevere food restriction (FR) impairs cardiac performance, although the causative mechanisms remain elusive. Since proteins associated with calcium handling may contribute to cardiac dysfunction, this study aimed to evaluate whether severe FR results in alterations in the expression and activity of Ca2+-handling proteins that contribute to impaired myocardial performance. Male 60-day-old Wistar-Kyoto rats were fed a control or restricted diet (50% reduction in the food consumed by the control group) for 90 days. Body weight, body fat pads, adiposity index, as well as the weights of the soleus muscle and lung, were obtained. Cardiac remodeling was assessed by morphological measures. The myocardial contractile performance was analyzed in isolated papillary muscles during the administration of extracellular Ca2+ and in the absence or presence of a sarcoplasmic reticulum Ca2+-ATPase (SERCA2a) specific blocker. The expression of Ca2+-handling regulatory proteins was analyzed via Western Blot. Severe FR resulted in a 50% decrease in body weight and adiposity measures. Cardiac morphometry was substantially altered, as heart weights were nearly twofold lower in FR rats. Papillary muscles isolated from FR hearts displayed mechanical dysfunction, including decreased developed tension and reduced contractility and relaxation. The administration of a SERCA2a blocker led to further decrements in contractile function in FR hearts, suggesting impaired SERCA2a activity. Moreover, the FR rats presented a lower expression of L-type Ca2+ channels. Therefore, myocardial dysfunction induced by severe food restriction is associated with changes in the calcium-handling properties in rats.en
dc.description.affiliationSao Paulo State Univ, Botucatu Med Sch, Dept Internal Med, BR-18618687 Botucatu, SP, Brazil
dc.description.affiliationUniv Fed Espirito Santo, Ctr Phys Educ & Sports, Dept Sports, BR-29075910 Vitoria, ES, Brazil
dc.description.affiliationSao Paulo State Univ, Inst Biosci, Dept Biostat, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUrsinus Coll, Dept Hlth & Exercise Physiol, Collegeville, PA 19426 USA
dc.description.affiliationUnespSao Paulo State Univ, Botucatu Med Sch, Dept Internal Med, BR-18618687 Botucatu, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Inst Biosci, Dept Biostat, BR-18618970 Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2009/52862-0
dc.description.sponsorshipIdFAPESP: 2010/50961-9
dc.format.extent12
dc.identifierhttp://dx.doi.org/10.3390/nu11091985
dc.identifier.citationNutrients. Basel: Mdpi, v. 11, n. 9, 12 p., 2019.
dc.identifier.doi10.3390/nu11091985
dc.identifier.urihttp://hdl.handle.net/11449/196202
dc.identifier.wosWOS:000487964600065
dc.language.isoeng
dc.publisherMdpi
dc.relation.ispartofNutrients
dc.sourceWeb of Science
dc.subjectmalnutrition
dc.subjectheart impairment
dc.subjectpapillary muscle assay
dc.subjectcalcium transient proteins
dc.subjectSERCA2a
dc.subjectL-type calcium channel
dc.titleMyocardial Dysfunction after Severe Food Restriction Is Linked to Changes in the Calcium-Handling Properties in Ratsen
dc.typeArtigo
dcterms.rightsHolderMdpi
unesp.author.lattes8727897080522289[11]
unesp.author.orcid0000-0002-4402-6523[13]
unesp.author.orcid0000-0002-7719-9682[11]

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Artigos - Clínica Médica - FMB
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