Modifying effect of propolis on dimethylhydrazine-induced DNA damage but not colonic aberrant crypt foci in rats

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dc.contributor.authorde Lima, ROA
dc.contributor.authorBazo, A. P.
dc.contributor.authorSaid, R. A.
dc.contributor.authorSforcin, José Mauricio [UNESP]
dc.contributor.authorBankova, V
dc.contributor.authorDarros, B. R.
dc.contributor.authorSalvadori, Daisy Maria Favero [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Estadual Santa Cruz
dc.contributor.institutionBulgarian Acad Sci
dc.date.accessioned2014-05-20T13:37:07Z
dc.date.available2014-05-20T13:37:07Z
dc.date.issued2005-01-01
dc.description.abstractPropolis is a honeybee product with several biological and therapeutic properties, including antimutagenic and anticarcinogenic activities. The effects of an aqueous extract of propolis (AEP) were evaluated on the formation of 1,2-dimethylhydrazine (DMH)-induced aberrant crypt foci (ACF) and DNA damage in the colon of male Wistar rats by the ACF and Comet assays, respectively. AEP was administered orally at 0.01%, 0.03%, 0.1%, and 0.3% in the drinking water, which resulted in doses of approximately 12, 34, 108, and 336 mg/kg body weight/day. Animals were also given a single subcutaneous injection of 40 mg/kg DMH and sacrificed 4 hr later for evaluating DNA damage, or 4 doses of 40 mg/kg DMH, administered 2 doses/week for 2 weeks, and sacrificed 12 weeks after the last injection for evaluating ACF development in the distal colon. Administration of AEP either simultaneously with or after the DMH treatment resulted in no statistically significant reduction of ACF. In contrast, 0.01%, 0.03%, and 0.3% AEP, given simultaneously with DMH, reduced DNA damage induction in the mid and distal colon. However, 0.3% AEP alone increased DNA damage in the colon. In conclusion, AEP had no effect on the formation of DMH-induced ACF in rat colon, but it modulated DMH-induced DNA damage in colon cells. Further investigations are recommended in order to establish the conditions under which propolis produces either protective or deleterious effects. (C) 2004 Wiley-Liss, Inc.en
dc.description.affiliationUniv Estadual Paulista Julio Mesquita Filho, Fac Med, Dept Patol, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUniv Estadual Paulista Julio Mesquita Filho, Inst Biociencias, Dept Microbiol & Imunol, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUniv Estadual Santa Cruz, Fac Ciências Agr & Ambientais, Ilheus, Bahia, Brazil
dc.description.affiliationBulgarian Acad Sci, Inst Organ Chem, Ctr Phytochem, Sofia, Bulgaria
dc.description.affiliationUnespUniv Estadual Paulista Julio Mesquita Filho, Fac Med, Dept Patol, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista Julio Mesquita Filho, Inst Biociencias, Dept Microbiol & Imunol, BR-18618000 Botucatu, SP, Brazil
dc.format.extent8-16
dc.identifierhttp://dx.doi.org/10.1002/em.20082
dc.identifier.citationEnvironmental and Molecular Mutagenesis. Hoboken: Wiley-liss, v. 45, n. 1, p. 8-16, 2005.
dc.identifier.doi10.1002/em.20082
dc.identifier.issn0893-6692
dc.identifier.lattes5051118752980903
dc.identifier.urihttp://hdl.handle.net/11449/12824
dc.identifier.wosWOS:000226735200002
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofEnvironmental and Molecular Mutagenesis
dc.relation.ispartofjcr3.254
dc.relation.ispartofsjr1,119
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectaberrant crypt focipt
dc.subjectanticarcinogenesispt
dc.subjectantimutagenesispt
dc.subjectchemopreventionpt
dc.subjectcomet assaypt
dc.subjectpropolispt
dc.titleModifying effect of propolis on dimethylhydrazine-induced DNA damage but not colonic aberrant crypt foci in ratsen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-Blackwell
unesp.author.lattes5051118752980903
unesp.author.orcid0000-0001-9323-3134[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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Artigos - Patologia - FMB
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