Comparison of microRNA Expression Profile in Chronic Myeloid Leukemia Patients Newly Diagnosed and Treated by Allogeneic Hematopoietic Stem Cell Transplantation

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dc.contributor.authorMartins, Juliana Ravelli Baldassarre [UNESP]
dc.contributor.authorMoraes, Leonardo Nazario de [UNESP]
dc.contributor.authorCury, Sarah Santiloni [UNESP]
dc.contributor.authorDadalto, Juliane [UNESP]
dc.contributor.authorCapannacci, Juliana [UNESP]
dc.contributor.authorCarvalho, Robson Francisco [UNESP]
dc.contributor.authorNogueira, Célia Regina [UNESP]
dc.contributor.authorHokama, Newton Key [UNESP]
dc.contributor.authorHokama, Paula de Oliveira Montandon [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2021-06-25T10:33:45Z
dc.date.available2021-06-25T10:33:45Z
dc.date.issued2020-09-04
dc.description.abstractChronic myeloid leukemia (CML) results from a translocation between chromosomes 9 and 22, which generates the Philadelphia chromosome. This forms BCR/ABL1, an active tyrosine kinase protein that promotes cell growth and replication. Despite great progress in CML treatment in the form of tyrosine kinase inhibitors, allogeneic-hematopoietic stem cell transplantation (allo-HSCT) is currently used as an important treatment alternative for patients resistant to these inhibitors. Studies have shown that unregulated expression of microRNAs, which act as oncogenes or tumor suppressors, is associated with human cancers. This contributes to tumor formation and development by stimulating proliferation, angiogenesis, and invasion. Research has demonstrated the potential of microRNAs as biomarkers for cancer diagnosis, prognosis, and therapeutic targets. In the present study, we compared the circulating microRNA expression profiles of 14 newly diagnosed patients with chronic phase-CML and 14 Philadelphia chromosome-negative patients after allo-HSCT. For each patient, we tested 758 microRNAs by reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis. The global expression profile of microRNAs revealed 16 upregulated and 30 downregulated microRNAs. Target genes were analyzed, and key pathways were extracted and compared. Bioinformatics tools were used to analyze data. Among the downregulated miRNA target genes, some genes related to cell proliferation pathways were identified. These results reveal the comprehensive microRNA profile of CML patients and the main pathways related to the target genes of these miRNAs in cytogenetic remission after allo-HSCT. These results provide new resources for exploring stem cell transplantation-based CML treatment strategies.en
dc.description.affiliationDepartment of Internal Medicine São Paulo State University (UNESP-FMB)
dc.description.affiliationDepartment of Bioprocesses and Biotechnology São Paulo State University (UNESP-FCA)
dc.description.affiliationDepartment of Structural and Functional Biology São Paulo State University (UNESP-IBB)
dc.description.affiliationUnespDepartment of Internal Medicine São Paulo State University (UNESP-FMB)
dc.description.affiliationUnespDepartment of Bioprocesses and Biotechnology São Paulo State University (UNESP-FCA)
dc.description.affiliationUnespDepartment of Structural and Functional Biology São Paulo State University (UNESP-IBB)
dc.identifierhttp://dx.doi.org/10.3389/fonc.2020.01544
dc.identifier.citationFrontiers in Oncology, v. 10.
dc.identifier.doi10.3389/fonc.2020.01544
dc.identifier.issn2234-943X
dc.identifier.scopus2-s2.0-85091229441
dc.identifier.urihttp://hdl.handle.net/11449/206531
dc.language.isoeng
dc.relation.ispartofFrontiers in Oncology
dc.sourceScopus
dc.subjectallogeneic hematopoietic stem cell transplantation
dc.subjectbiomarkers
dc.subjectchronic myeloid leukemia
dc.subjectmiRNAs
dc.subjectPhiladelphia chromosome
dc.titleComparison of microRNA Expression Profile in Chronic Myeloid Leukemia Patients Newly Diagnosed and Treated by Allogeneic Hematopoietic Stem Cell Transplantationen
dc.typeArtigo

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