Progesterone dose during synchronization treatment alters luteinizing hormone receptor and steroidogenic enzyme mRNA abundances in granulosa cells of Nellore heifers

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dc.contributor.authorDias, H. P. [UNESP]
dc.contributor.authorPoole, R. K.
dc.contributor.authorAlbuquerque, J. P. [UNESP]
dc.contributor.authorSantos, P. H. dos [UNESP]
dc.contributor.authorCastilho, A. C. S.
dc.contributor.authorPohler, K. G.
dc.contributor.authorVasconcelos, J. L. M. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionTexas A&M Univ
dc.contributor.institutionUniv Western Sao Paulo
dc.date.accessioned2021-06-25T12:34:43Z
dc.date.available2021-06-25T12:34:43Z
dc.date.issued2021-02-01
dc.description.abstractThe objective was to investigate effects of progesterone (P-4) dose on abundance of luteinizing hormone receptor (LHCGR), aromatase (CYP19A1), 3 beta-hydroxysteroid dehydrogenase (HSD3B1), and other steroidogenic mRNA transcripts in granulosa cells from dominant follicles. Nellore heifers were assigned to one of six groups: new, first-use controlled internal drug release device (CIDR1) inserted for 5 days (Large-P-4-dose-D5; n = 7) or 6 days (Large-P-4-dose-D6; n = 8), prostaglandin (PG)F-2 alpha administered on DO and 1 previously-used CIDR (CIDR3) inserted for 5 days (Small-P-4-dose-D5; n = 8) or 6 days (Small-P-4-dose-D6; n = 8), CIDR1 inserted on DO and removed plus PGF(2 alpha) on D5 (Large-P-4-dose-pmestrus (PE); n = 7), and CIDR3 and PGF(2 alpha) on DO and 1, CIDR3 removed plus PGF(2 alpha) on D5 (Small-P-4-dose-PE; n = 7). Duration of P-4 treatment (D5 compared to D6) affected abundances of CYP19A1 mRNA transcripts, with there being greater abundances on D6 than D5 (P <= 0.05). Heifers treated with the large dose of P-4 had a smaller dominant follicle, less serum and intra-follicular estradiol (E-2) concentrations (P <= 0.05) and lesser LHCGR, CYP19A1, and HSD3B1 transcript abundances (P <= 0.05). Heifers treated to induce PE had a larger follicle diameter (P = 0.09), greater intra-follicular E-2 concentrations and larger abundances of CYP19A1 mRNA transcript (P <= 0.05) than heifers of the D6 group. Overall, treatment with larger doses of P-4 resulted in lesser abundances of LHCGR, HSD3B1, and CYP19A1 mRNA transcripts; thus, potentially leading to development of smaller dominant follicles and lesser E-2 concentrations.en
dc.description.affiliationSao Paulo State Univ, Dept Anim Prod, BR-18168000 Botucatu, SP, Brazil
dc.description.affiliationTexas A&M Univ, Dept Anim Sci, Pregnancy & Dev Programming Area Excellence, College Stn, TX 77843 USA
dc.description.affiliationSao Paulo State Univ, Inst Biosci, BR-18168000 Botucatu, SP, Brazil
dc.description.affiliationUniv Western Sao Paulo, Presidente Prudente, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Dept Anim Prod, BR-18168000 Botucatu, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Inst Biosci, BR-18168000 Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipUSDA-NIFAHatch/Multistate Project
dc.description.sponsorshipIdFAPESP: 2013/17181-8
dc.description.sponsorshipIdUSDA-NIFAHatch/Multistate Project: W3112-TEX07702
dc.format.extent10
dc.identifierhttp://dx.doi.org/10.1016/j.anireprosci.2020.106681
dc.identifier.citationAnimal Reproduction Science. Amsterdam: Elsevier, v. 225, 10 p., 2021.
dc.identifier.doi10.1016/j.anireprosci.2020.106681
dc.identifier.issn0378-4320
dc.identifier.urihttp://hdl.handle.net/11449/209952
dc.identifier.wosWOS:000610778500007
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofAnimal Reproduction Science
dc.sourceWeb of Science
dc.subjectLH receptor
dc.subjectProgesterone
dc.subjectGranulosa cell
dc.subjectNellore heifers
dc.titleProgesterone dose during synchronization treatment alters luteinizing hormone receptor and steroidogenic enzyme mRNA abundances in granulosa cells of Nellore heifersen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.

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