Treatment of chronic spinal cord injury in dogs using amniotic membrane-derived stem cells: Preliminary results

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dc.contributor.authorOrlandin, Jéssica Rodrigues
dc.contributor.authorGomes, Ingrid da Silva
dc.contributor.authorLeandro, Shamira de Fátima Sallum
dc.contributor.authorCagnim, Artur Fuertes
dc.contributor.authorCasals, Juliana Barbosa
dc.contributor.authorCarregaro, Adriano Bonfim
dc.contributor.authorFreitas, Silvio Henrique
dc.contributor.authorMachado, Luciana Cristina
dc.contributor.authorCastiglioni, Maria Cristina Reis [UNESP]
dc.contributor.authorAlves, Ana Liz Garcia [UNESP]
dc.contributor.authorMachado, Vânia Maria de Vasconcelos [UNESP]
dc.contributor.authorAmbrósio, Carlos Eduardo
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-05-01T09:47:29Z
dc.date.available2022-05-01T09:47:29Z
dc.date.issued2021-01-01
dc.description.abstractIntroduction: Intervertebral disc diseases (IVDD) represent the majority of neurological attendance and responsible for the most cases of paralysis in dogs. Treatments currently used do not show satisfactory results in patients with more severe and chronic neurological manifestations. Methods: To promote nerve and muscular recovery, as well as improve quality of life, we aimed to create a double-blind test method, associating spinal decompression surgery and allogeneic transplantation of amniotic membrane-derived stem cells (AMSCs) in dogs with chronic IVDD. Cells were characterized as fetal mesenchymal cells and safe for application. Eight animals completed the experiment: stem cell applications were made in four animals that had previously undergone an unsuccessful surgical procedure (“SC group”, n = 4); two animals were submitted to surgery, followed by applications of stem cells (“Surgery + SC”, n = 2); two other animals were submitted to surgery, followed by the application of saline solution (“Surgery + placebo”, n = 2). During the surgical procedure, a topical application was performed on the lesion and after fifteen and forty-five days another two applications were made via epidural. Animals were monitored biweekly and reassessed three months after surgery, by functional tests and magnetic resonance exams. Results: Some animals presented significant neurological improvement, such as the recovery of nociception and ability to remain on station. Despite the need further studies, until the present moment, cell therapy has been feasible and has no harmful effects on animals. Conclusion: The protocol of preclinical trial showed the association with decompressive surgery and cell transplantation in dogs with thoracolumbar IVDD proved feasible, and it was possible to observe neurological improvement after treatment. No tissue improvement through MRI was found. The double-blind test guaranteed reliability of the evaluations and results obtained that, even with a small sample size, generated satisfactory results for the animals and owners.en
dc.description.affiliationDepartment of Veterinary Medicine Faculty of Animal Science and Food Engineering (FZEA-USP) University of São Paulo
dc.description.affiliationDepartment of Veterinary Medicine School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Veterinary Medicine School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2009/54028-8
dc.description.sponsorshipIdFAPESP: 2017/21266-0
dc.format.extent39-49
dc.identifierhttp://dx.doi.org/10.2147/SCCAA.S324184
dc.identifier.citationStem Cells and Cloning: Advances and Applications, v. 14, p. 39-49.
dc.identifier.doi10.2147/SCCAA.S324184
dc.identifier.issn1178-6957
dc.identifier.scopus2-s2.0-85117847703
dc.identifier.urihttp://hdl.handle.net/11449/233738
dc.language.isoeng
dc.relation.ispartofStem Cells and Cloning: Advances and Applications
dc.sourceScopus
dc.subjectAmnion
dc.subjectCell therapy
dc.subjectDiscopathy
dc.subjectRegenerative medicine
dc.subjectSpinal cord injury
dc.titleTreatment of chronic spinal cord injury in dogs using amniotic membrane-derived stem cells: Preliminary resultsen
dc.typeArtigo

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