Effect of lignin content on cellulolytic saccharification of liquid hot water pretreated sugarcane bagasse

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dc.contributor.authorLadeira Ázar, Rafaela I.S.
dc.contributor.authorBordignon-Junior, Sidnei Emilio [UNESP]
dc.contributor.authorLaufer, Craig
dc.contributor.authorSpecht, Jordan
dc.contributor.authorFerrier, Drew
dc.contributor.authorKim, Daehwan
dc.contributor.institutionFederal University of Viçosa
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionHood College
dc.date.accessioned2020-12-12T01:55:38Z
dc.date.available2020-12-12T01:55:38Z
dc.date.issued2020-01-31
dc.description.abstractLignin contributes to the rigid structure of the plant cell wall and is partially responsible for the recalcitrance of lignocellulosic materials to enzymatic digestion. Overcoming this recalcitrance is one the most critical issues in a sugar-flat form process. This study addresses the effect of low lignin sugarcane bagasse on enzymatic hydrolysis after liquid hot water pretreatment at 190 ◦C and 20 min (severity factor: 3.95). The hydrolysis of bagasse from a sugarcane line selected for a relatively low lignin content, gave an 89.7% yield of cellulose conversion to glucose at 40 FPU/g glucan versus a 68.3% yield from a comparably treated bagasse from the high lignin bred line. A lower enzyme loading of 5 FPU/g glucan (equivalent to 3.2 FPU/g total solids) resulted in 31.4% and 21.9% conversion yields, respectively, for low and high lignin samples, suggesting the significance of lignin content in the saccharification process. Further increases in the enzymatic conversion of cellulose to glucose were achieved when the bagasse sample was pre-incubated with a lignin blocking agent, e.g., bovine serum albumin (50 mg BSA/g glucan) at 50 ◦C for 1 h prior to an actual saccharification. In this work, we have demonstrated that even relatively small differences in lignin content can result in considerably increased sugar production, which supports the dissimilarity of bagasse lignin content and its effects on cellulose digestibility. The increased glucose yields with the addition of BSA helped to decrease the inhibition of non-productive absorption of cellulose enzymes onto lignin and solid residual lignin fractions.en
dc.description.affiliationDepartment of Biochemistry and Molecular Biology BIOAGRO Federal University of Viçosa
dc.description.affiliationLaboratory of Biochemistry and Applied Microbiology São Paulo State University (UNESP) IBILCE, 2265 Cristóvão Colombo
dc.description.affiliationDepartment of Biology Hood College, 401 Rosemont Avenue
dc.description.affiliationUnespLaboratory of Biochemistry and Applied Microbiology São Paulo State University (UNESP) IBILCE, 2265 Cristóvão Colombo
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipInnovative Medicines Initiative
dc.description.sponsorshipBard Summer Research Institute, Bard College
dc.identifierhttp://dx.doi.org/10.3390/molecules25030623
dc.identifier.citationMolecules, v. 25, n. 3, 2020.
dc.identifier.doi10.3390/molecules25030623
dc.identifier.issn1420-3049
dc.identifier.scopus2-s2.0-85078903720
dc.identifier.urihttp://hdl.handle.net/11449/200027
dc.language.isoeng
dc.relation.ispartofMolecules
dc.sourceScopus
dc.subjectBovine serum albumin
dc.subjectEnzymatic hydrolysis
dc.subjectInhibition
dc.subjectLignin
dc.subjectSugarcane bagasse
dc.titleEffect of lignin content on cellulolytic saccharification of liquid hot water pretreated sugarcane bagasseen
dc.typeArtigo

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