Investigation of canine visceral leishmaniasis in a non-endemic area in Brazil and the comparison of serological and molecular diagnostic tests

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dc.contributor.authorSevá, Anaiá da Paixão
dc.contributor.authorBrandão, Ana Pérola Drulla
dc.contributor.authorGodoy, Silvia Neri
dc.contributor.authorSoares, Rodrigo Martins
dc.contributor.authorLangoni, Helio [UNESP]
dc.contributor.authorRodrigues, Bruna Cristine
dc.contributor.authorGava, Mariana Zanchetta E [UNESP]
dc.contributor.authorZanotto, Paula Ferraz de Camargo [UNESP]
dc.contributor.authorJimenez-Villegas, Tatiana
dc.contributor.authorHiramoto, Roberto
dc.contributor.authorFerreira, Fernando
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionInstituto Chico Mendez de Conservação da Biodiversidade
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionInstituto Adolfo Lutz
dc.date.accessioned2022-05-01T09:30:55Z
dc.date.available2022-05-01T09:30:55Z
dc.date.issued2021-01-01
dc.description.abstractINTRODUCTION: Visceral leishmaniasis (VL) is an important zoonosis in Brazil. Previous identification of parasitized dogs can also help prevent the disease in humans, even in non-endemic areas of the country. The Brazilian Ministry of Health recommends diagnosis in dogs using a DPP® (rapid test) as a screening test and an immunoenzymatic assay (ELISA) as a confirmatory test (DPP®+ELISA), and culling infected dogs as a legal control measure. However, the accuracy of these serological tests has been questioned. METHODS: VL in dogs was investigated in a non-endemic area of the São Paulo state for three consecutive years, and the performances of different diagnostic tests were compared. RESULTS: A total of 331 dog samples were collected in 2015, 373 in 2016, and 347 in 2017. The seroprevalence by DPP®+ELISA was 3.3, 3.2, and 0.3%, respectively, and by indirect immunofluorescence assay (IFA), it was 3.0, 5.6, and 5.5%, respectively. ELISA confirmed 18.4% of DPP® positive samples. The concordance between the IFA and DPP® was 83.9%. The concordance between IFA and DPP®+ELISA was 92.9%. A molecular diagnostic test (PCR) was performed in 63.2% of the seropositive samples, all of which were negative. CONCLUSIONS: In non-endemic areas, diagnostic tests in dogs should be carefully evaluated to avoid false results.en
dc.description.affiliationUniversidade de São Paulo Departamento de Veterinária Preventiva e Saúde Animal
dc.description.affiliationUniversidade Estadual de Santa Cruz, Departamento de Ciências Agrárias e Ambientais, Ilhéus, BA, Brasil
dc.description.affiliationInstituto Chico Mendez de Conservação da Biodiversidade
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho Departamento de Higiene Veterinária e Saúde Pública
dc.description.affiliationInstituto Adolfo Lutz
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho Departamento de Higiene Veterinária e Saúde Pública
dc.format.extente01822021
dc.identifierhttp://dx.doi.org/10.1590/0037-8682-0182-2021
dc.identifier.citationRevista da Sociedade Brasileira de Medicina Tropical, v. 54, p. e01822021-.
dc.identifier.doi10.1590/0037-8682-0182-2021
dc.identifier.issn1678-9849
dc.identifier.scopus2-s2.0-85115931966
dc.identifier.urihttp://hdl.handle.net/11449/233600
dc.language.isoeng
dc.relation.ispartofRevista da Sociedade Brasileira de Medicina Tropical
dc.sourceScopus
dc.titleInvestigation of canine visceral leishmaniasis in a non-endemic area in Brazil and the comparison of serological and molecular diagnostic testsen
dc.typeArtigo
unesp.author.orcid0000-0001-5203-012X 0000-0001-5203-012X[1]
unesp.author.orcid0000-0001-6783-0242[2]
unesp.author.orcid0000-0003-3041-4389[3]
unesp.author.orcid0000-0002-7736-7914[4]
unesp.author.orcid0000-0001-5127-0762[5]
unesp.author.orcid0000-0002-7522-8152[6]
unesp.author.orcid0000-0001-6342-2073[7]
unesp.author.orcid0000-0002-1932-3702[8]
unesp.author.orcid0000-0001-6496-2418[9]
unesp.author.orcid0000-0002-7404-1505[10]
unesp.author.orcid0000-0002-9160-7355[11]

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Artigos - Higiene Veterinária e Saúde Pública - FMVZ