The aggregate-forming pili (AFP) mediates the aggregative adherence of a hybrid-pathogenic Escherichia coli (UPEC/EAEC) isolated from a urinary tract infection

Página do item simplificado
dc.contributor.authorSchüroff, Paulo A.
dc.contributor.authorSalvador, Fábia A.
dc.contributor.authorAbe, Cecilia M.
dc.contributor.authorWami, Haleluya T.
dc.contributor.authorCarvalho, Eneas
dc.contributor.authorHernandes, Rodrigo T. [UNESP]
dc.contributor.authorDobrindt, Ulrich
dc.contributor.authorGomes, Tânia A. T.
dc.contributor.authorElias, Waldir P.
dc.contributor.institutionInstituto Butantan
dc.contributor.institutionUniversity of Münster
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-29T08:46:12Z
dc.date.available2022-04-29T08:46:12Z
dc.date.issued2021-01-01
dc.description.abstractEnteroaggregative Escherichia coli (EAEC) comprises an important diarrheagenic pathotype, while uropathogenic E. coli (UPEC) is the most important agent of urinary tract infection (UTI). Recently, EAEC virulence factors have been detected in E. coli strains causing UTI, showing the importance of these hybrid-pathogenic strains. Previously, we detected an E. coli strain isolated from UTI (UPEC-46) presenting characteristics of EAEC, e.g., the aggregative adherence (AA) pattern and EAEC-associated genes (aatA, aap, and pet). In this current study, we analyzed the whole genomic sequence of UPEC-46 and characterized some phenotypic traits. The AA phenotype was observed in cell lineages of urinary and intestinal origin. The production of curli, cellulose, bacteriocins, and Pet toxin was detected. Additionally, UPEC-46 was not capable of forming biofilm using different culture media and human urine. The genome sequence analysis showed that this strain belongs to serotype O166:H12, ST10, and phylogroup A, harbors the tet, aadA, and dfrA/sul resistance genes, and is phylogenetically more related to EAEC strains isolated from human feces. UPEC-46 harbors three plasmids. Plasmid p46-1 (~135 kb) carries some EAEC marker genes and those encoding the aggregate-forming pili (AFP) and its regulator (afpR). A mutation in afpA (encoding the AFP major pilin) led to the loss of pilin production and assembly, and notably, a strongly reduced adhesion to epithelial cells. In summary, the genetic background and phenotypic traits analyzed suggest that UPEC-46 is a hybrid strain (UPEC/EAEC) and highlights the importance of AFP adhesin in the adherence to colorectal and bladder cell lines.en
dc.description.affiliationLaboratório de Bacteriologia Instituto Butantan
dc.description.affiliationInstitute of Hygiene University of Münster
dc.description.affiliationDepartamento de Microbiologia Imunologia e Parasitologia Escola Paulista de Medicina Universidade Federal de São Paulo
dc.description.affiliationInstituto de Biociências Universidade Estadual Paulista (UNESP)
dc.description.affiliationUnespInstituto de Biociências Universidade Estadual Paulista (UNESP)
dc.format.extent3073-3093
dc.identifierhttp://dx.doi.org/10.1080/21505594.2021.2007645
dc.identifier.citationVirulence, v. 12, n. 1, p. 3073-3093, 2021.
dc.identifier.doi10.1080/21505594.2021.2007645
dc.identifier.issn2150-5608
dc.identifier.issn2150-5594
dc.identifier.scopus2-s2.0-85121522549
dc.identifier.urihttp://hdl.handle.net/11449/231573
dc.language.isoeng
dc.relation.ispartofVirulence
dc.sourceScopus
dc.subjectaggregate-forming pilus
dc.subjectEAEC
dc.subjecthybrid-pathogenic E. coli
dc.subjectUPEC
dc.subjectUTI
dc.titleThe aggregate-forming pili (AFP) mediates the aggregative adherence of a hybrid-pathogenic Escherichia coli (UPEC/EAEC) isolated from a urinary tract infectionen
dc.typeArtigo
unesp.author.orcid0000-0002-7808-7818[1]
unesp.author.orcid0000-0003-0218-9372[3]
unesp.author.orcid0000-0002-9929-2570[4]
unesp.author.orcid0000-0002-8052-0975[5]
unesp.author.orcid0000-0001-6695-6003[6]
unesp.author.orcid0000-0001-9949-1898[7]
unesp.author.orcid0000-0002-4525-8705[8]
unesp.author.orcid0000-0003-3470-5706[9]

Arquivos

Coleções

Artigos - Parasitologia - IBB