From the dual cyclone harvest performance of single conidium powder to the effect of Metarhizium anisopliae on the management of Thaumastocoris peregrinus (Hemiptera: Thaumastocoridae)

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dc.contributor.authorVelozo, Simone Graziele Moio [UNESP]
dc.contributor.authorVelozo, Murilo Rodrigues
dc.contributor.authorDomingues, Maurício Magalhães [UNESP]
dc.contributor.authorBecchi, Luciane Katarine [UNESP]
dc.contributor.authorde Carvalho, Vanessa Rafaela [UNESP]
dc.contributor.authorde Souza Passos, José Raimundo [UNESP]
dc.contributor.authorZanuncio, José Cola
dc.contributor.authorSerrão, José Eduardo
dc.contributor.authorStephan, Dietrich
dc.contributor.authorWilcken, Carlos Frederico [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Federal de Viçosa (UFV)
dc.contributor.institutionSyntech Research
dc.contributor.institutionFederal Research Centre for Cultivated Plants
dc.date.accessioned2023-07-29T13:47:49Z
dc.date.available2023-07-29T13:47:49Z
dc.date.issued2023-03-01
dc.description.abstractInsect pests introduced in eucalyptus plantations in Brazil are mostly of Australian origin, but native microorganisms have potential for their management. High quality biopesticide production based on entomopathogenic fungi depends on adequate technologies. The objective of this study was to evaluate Mycoharvester® equipment to harvest and separating particles to obtain pure Metarhizium anisopliae conidia to manage Thaumastocoris peregrinus Carpintero & Dellapé, 2006 (Hemiptera: Thaumastocoridae). The Mycoharvester® version 5b harvested and separated M. anisopliae spores. The pure conidia were suspended in Tween 80® (0.1%) and calibrated to the concentrations of 1 x 106, 107, 108 and 109 conidia/ ml to evaluate the pathogenicity, lethal concentration 50 and 90 (LC50, LC90) and lethal time 50 and 90 (LT50, LT90) of this fungus to T. peregrinus. This equipment harvested 85% of the conidia from rice, with production of 4.8 ± 0.38 x 109 conidia/g dry mass of substrate + fungus. The water content of 6.36% of the single spore powder (pure conidia) separated by the Mycoharvester® was lower than that of the agglomerated product. The product harvested at the concentrations of 108 and 109 conidia/ml caused high mortality to T. peregrinus third instar nymphs and adults. The separation of conidia produced by solid-state fermentation with the Mycoharvester® is an important step toward optimizing the fungal production system of pure conidia, and to formulate biopesticides for insect pest management.en
dc.description.affiliationFaculdade de Ciências Agronômicas Laboratório de Controle Biológico de Pragas Florestais Universidade Estadual Paulista (UNESP), São Paulo
dc.description.affiliationDepartamento de Biologia Geral Universidade Federal de Viçosa, Minas Gerais
dc.description.affiliationSyntech Research, São Paulo
dc.description.affiliationDepartamento de Bioestatística Instituto de Biociências Universidade Estadual Paulista (UNESP), São Paulo
dc.description.affiliationDepartamento de Entomologia BIOAGRO Universidade Federal de Viçosa, Minas Gerais
dc.description.affiliationJulius Kühn-Institut Institute for Biological Control Federal Research Centre for Cultivated Plants
dc.description.affiliationUnespFaculdade de Ciências Agronômicas Laboratório de Controle Biológico de Pragas Florestais Universidade Estadual Paulista (UNESP), São Paulo
dc.description.affiliationUnespDepartamento de Bioestatística Instituto de Biociências Universidade Estadual Paulista (UNESP), São Paulo
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdCAPES: 001
dc.description.sponsorshipIdCAPES: 88881.134760/2016-01
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0283543
dc.identifier.citationPLoS ONE, v. 18, n. 3 March, 2023.
dc.identifier.doi10.1371/journal.pone.0283543
dc.identifier.issn1932-6203
dc.identifier.scopus2-s2.0-85150961608
dc.identifier.urihttp://hdl.handle.net/11449/248577
dc.language.isoeng
dc.relation.ispartofPLoS ONE
dc.sourceScopus
dc.titleFrom the dual cyclone harvest performance of single conidium powder to the effect of Metarhizium anisopliae on the management of Thaumastocoris peregrinus (Hemiptera: Thaumastocoridae)en
dc.typeArtigo

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