Matrix metalloproteinase (MMP)-2 and MMP-9 activity and localization during ventral prostate atrophy and regrowth

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dc.contributor.authorJustulin, L. A. [UNESP]
dc.contributor.authorDella-Coleta, H. H. M.
dc.contributor.authorTaboga, S. R. [UNESP]
dc.contributor.authorFelisbino, S. L. [UNESP]
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.date.accessioned2014-05-20T14:01:03Z
dc.date.available2014-05-20T14:01:03Z
dc.date.issued2010-10-01
dc.description.abstractMatrix metalloproteinses (MMPs) are enzymes involved in prostatic development, growth, disease-induced tissue remodelling and secretory fluid. Although the prostate function depends upon androgen regulation, the relationship between MMPs and androgen has not been well established. Here, we evaluated MMP-2 and MMP-9 gelatinolytic activity in association with tissue localization during ventral prostate atrophy and regrowth induced by testosterone replacement (TR). Adult male Wistar rats were divided into three experimental groups: control, castrated (CS) and TR 21 days after castration. Ventral prostate (VP) was excised at 3, 5, 7 and 21 days after castration in CS group, and at 3, 5, 7 and 10 days after TR (4 mg/kg/day) in TR group. The VP was dissected, weighed and processed for histology, immunohistochemistry, ultrastructure and zymography analyses. Castration elicited the typical parenchymal atrophy and stromal condensation. TR induced intense epithelial growth towards the stromal space to restore the prostate histoarchitecture. MMP-2 and MMP-9 immunostaining presented intense reaction in CS and TR groups, mainly in the epithelial and endothelial cells. After TR, a strong immunoreaction for MMP-2 was observed in the activated stromal fibroblasts. Zymography showed that MMP-2 and MMP-9 activity, mainly the active form, increased after castration. In contrast, TR induced an additional increase in MMP-2 activity, but not in MMP-9. In conclusion, the overall behaviour of MMP-2 and MMP-9 within the prostate under androgen handling is highly complex, as each glandular compartment and cell type is affected differently by the androgenic status. Prostate regrowth appears to involve a more effective participation of MMP-2 in both epithelial and stromal compartments, while MMP-9 plays a major role in the late prostate atrophy and early regrowth.en
dc.description.affiliationUniv Estadual Campinas, Dept Cell Biol, Inst Biol, São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Morphol, Inst Biosci, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de Minas Gerais (UFMG), Dept Morphol, Inst Biol Sci, Belo Horizonte, MG, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Biol, Inst Biosci Humanities & Exact Sci, São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Morphol, Inst Biosci, São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Biol, Inst Biosci Humanities & Exact Sci, São Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação para o Desenvolvimento da UNESP (FUNDUNESP)
dc.description.sponsorshipIdFAPESP: 02/11102-4
dc.description.sponsorshipIdFAPESP: 04/08627-3
dc.description.sponsorshipIdCNPq: 476137/03-9
dc.format.extent696-708
dc.identifierhttp://dx.doi.org/10.1111/j.1365-2605.2009.01016.x
dc.identifier.citationInternational Journal of Andrology. Malden: Wiley-blackwell, v. 33, n. 5, p. 696-708, 2010.
dc.identifier.doi10.1111/j.1365-2605.2009.01016.x
dc.identifier.issn0105-6263
dc.identifier.lattes7263490918934874
dc.identifier.orcid0000-0002-0970-4288
dc.identifier.urihttp://hdl.handle.net/11449/21576
dc.identifier.wosWOS:000281555900004
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofInternational Journal of Andrology
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectandrogenen
dc.subjectcastrationen
dc.subjectcollagenen
dc.subjectextracellular matrixen
dc.subjectmatrix metalloproteinasesen
dc.subjectprostateen
dc.titleMatrix metalloproteinase (MMP)-2 and MMP-9 activity and localization during ventral prostate atrophy and regrowthen
dc.typeCarta
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-blackwell
unesp.author.lattes7263490918934874
unesp.author.orcid0000-0002-0970-4288[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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